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Croatica Chemica Acta, Vol. 81 No. 1, 2008.

Original scientific paper

Effects of Hyperglycemia and Metformin on Expression of Adhesion Molecules on Human Aortic Endothelial Cells

Tatjana Bačun orcid id orcid.org/0000-0001-7012-5325 ; Clinical Hospital Osijek, Osijek, Croatia
Tatjana Belovari ; School of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Mirjana Suver orcid id orcid.org/0000-0002-5418-3178 ; Clinical Hospital Osijek, Osijek, Croatia
Blaženka Dobrošević ; Clinical Hospital Osijek, Osijek, Croatia
Ljubica Glavaš-Obrovac orcid id orcid.org/0000-0001-7497-296X ; Clinical Hospital Osijek, Osijek, Croatia; School of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia

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Abstract

Expression of adhesion molecules on the endothelial cell surface as a response to elevated glucose concentration is considered as a main event in the development of atherosclerosis. The influence of high glucose concentration and metformin, a wide used anti-diabetic drug, on the expression of E-selectin, vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) on human aortic endothelial cells (HAECs) was investigated. HAECs were cultured 4 h in a medium with 5.5, 8.0, 12.0, and 16.5 mmol dm-3 glucose with or without metformin addition. The expression of cell adhesion molecules (CAM) was measured by flow-cytometry. Compared to CAM expression in the medium with 5.5 mmol dm-3 glucose, glucose concentration of 12.0 mmol dm-3 increased expression of E-selectin (62 %), VCAM-1 (four fold) and ICAM-1 (81 %). Metformin administration in the medium with 12.0 mmol dm-3 glucose additionally enhanced E-selectin and VCAM-1 expression compared to effects of corresponding glucose concentration. ICAM-1 expression was only significantly increased in the medium with metformin and 8.0 mmol dm-3 glucose. In conclusion, metformin in condition with elevated glucose concentration additionally increased expression of CAM on HAECs which could contribute to development of macrovascular complications in diabetic patients.

Keywords

cell adhesion molecules; endothelial cells; atherosclerosis; diabetes mellitus; metformin

Hrčak ID:

23442

URI

https://hrcak.srce.hr/23442

Publication date:

15.4.2008.

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