Original scientific paper

https://doi.org/10.24869/psyd.2019.249

ASSOCIATION OF NEUROPEPTIDE S RECEPTOR 1 AND GLUTAMATE DECARBOXYLASE 1 GENE POLYMORPHISMS WITH POSTTRAUMATIC STRESS DISORDER

Shpend Haxhibeqiri ; Institute of Kosovo Forensic Psychiatry, University Clinical Centre of Kosovo, Prishtina, Kosovo
Valdete Haxhibeqiri ; Department of Clinical Biochemistry, University Clinical Centre of Kosovo, Prishtina, Kosovo
Ferid Agani ; Faculty of Medicine, University Hasan Prishtina, Prishtina, Kosovo
Aferdita Goci Uka ; Department of Psychiatry, University Clinical Centre of Kosovo, Prishtina, Kosovo
Blerina Hoxha ; Department of Psychiatry, University Clinical Centre of Kosovo, Prishtina, Kosovo
Alma Dzubur Kulenovic ; Department of Psychiatric, Clinical Centre University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Abdulah Kučukalić ; Department of Psychiatric, Clinical Centre University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Esmina Avdibegović ; Department of Psychiatry, University Clinical Centre Tuzla, Tuzla, Bosnia and Herzegovina
Osman Sinanović ; Department of Psychiatry, University Clinical Centre Tuzla, Tuzla, Bosnia and Herzegovina
Dragan Babic ; Department of Psychiatry, University Clinical Centre of Mostar, Mostar, Bosnia and Herzegovina
Miro Jakovljevic ; Department of Psychiatry, University Hospital Centre Zagreb, Zagreb, Croatia
Sabina Kučukalić ; Department of Psychiatric, Clinical Centre University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Alma Bravo Mehmedbašić ; Department of Psychiatric, Clinical Centre University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Nermina Kravić ; Department of Psychiatry, University Clinical Centre Tuzla, Tuzla, Bosnia and Herzegovina
Mirnesa Muminović-Umihanić ; Department of Psychiatry, University Clinical Centre Tuzla, Tuzla, Bosnia and Herzegovina
Romana Babić ; Department of Psychiatry, University Clinical Centre of Mostar, Mostar, Bosnia and Herzegovina
Marko Pavlović ; Department of Psychiatry, University Clinical Centre of Mostar, Mostar, Bosnia and Herzegovina
Nenad Jakšić ; Department of Psychiatry, University Hospital Centre Zagreb, Zagreb, Croatia
Branka Aukst Margetić ; Department of Psychiatry, University Hospital Centre Sestre Milosrdnice, Zagreb, Croatia
Duško Rudan ; Department of Psychiatry, University Hospital Centre Zagreb, Zagreb, Croatia
Elma Ferić Bojić ; Department of Genetics and Bioengineering International Burch University, Sarajevo, Bosnia and Herzegovina
Damir Marjanović ; Department of Genetics and Bioengineering International Burch University, Sarajevo, Bosnia and Herzegovina
Christiane Ziegler ; Department of Psychiatry and Psychotherapy, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
Christiane Wolf ; Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital of Würzburg, Würzburg, Germany
Bodo Warrings ; Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital of Würzburg, Würzburg, Germany
Katharina Domschke ; Department of Psychiatry and Psychotherapy, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
Jürgen Deckert ; Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital of Würzburg, Würzburg, Germany

Abstract

Background: Posttraumatic stress disorder (PTSD) is an anxiety disorder caused by highly traumatic experiences. The aim of this study was to investigate the influence of single nucleotide polymorphisms (SNPs) in the neuropeptide S receptor 1 (NPSR1) and the glutamate decarboxylase 1(GAD1) gene on PTSD and its psychopathological aspects among individuals affected by the Balkan wars during the 90s.
Subjects and methods: This study was conducted as part of the South Eastern Europe (SEE) study on molecular mechanisms of PTSD. It comprised 719 participants (539 males), including those with current PTSD, remitted PTSD and healthy volunteers. Psychometric evaluation was performed using the Mini International Neuropsychiatric Interview (M.I.N.I.), the Clinician Administrated PTSD Scale (CAPS) andthe Brief Symptom Inventory (BSI). We examined NPSR1 single nucleotide polymorphism (SNP) rs324981 and GAD1 variant rs3749034 genotypes. Case-control analyses were carried out using logistical regression to determine genotype differences between all patients that had either current or remitted PTSD and control individuals. To analyse the
influence of the analysed SNPs on PTSD severity, we performed linear regression analyses with CAPS and BSI within each of the two patient groups separately. All of the calculations were performed for additive allelic, recessive, dominant and genotypic models.
Results: We observed a nominally significant association for the major allele (G) of GAD1 rs3749034 with an increased risk to develop PTSD in a case control analysis in the recessive model (P=0.0315, odds ratio=0.47, SE=0.35). In contrast, a nominally significant association of the minor allele (A) with higher CAPS scores was identified within the patient group with lifetime PTSD in the dominant model (P=0.0372, ????=6.29, SE=2.99). None of these results did withstand correction for multiple tests. No nominal significant results of GAD1 rs3749034 were found with regard to the intensity of psychological BSI symptoms. Case control analyses of NPSR1 rs324981 revealed a nominally significant higher risk for homozygous T allele carriers to develop PTSD
(P=0.0452) in the recessive model. On the other hand, the T allele showed a nominally significant association with higher BSI scores in patients suffering from lifetime PTSD in the recessive model (P=0.0434). Again, these results were not significant anymore after correction for multiple tests. No associations of NPSR1 rs324981 and CAPS score was identified.
Conclusion: The findings of this study provide some evidence that the NPSR1 and GAD1 polymorphisms might play a role in the development of war-related PTSD and its related psychological expressions. Further research is needed to elucidate the interactions of specific gene variants and environmental factors in the development of PTSD.

Keywords

PTSD; NPSR1; GAD1; gene polymorphism; war trauma

Hrčak ID:

235156

URI

https://hrcak.srce.hr/235156

Publication date:

9.7.2019.

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