Skip to the main content

Biochemia Medica, Vol. 30 No. 2, 2020.

Original scientific paper

https://doi.org/10.11613/BM.2020.020710

Verification of the Canadian Laboratory Initiative on Paediatric Reference Intervals (CALIPER) reference values in Croatian children and adolescents

Renata Zrinski Topić orcid id orcid.org/0000-0002-3740-7854 ; Department of Laboratory Diagnostics, Children’s Hospital Zagreb, Zagreb, Croatia
Jasna Leniček Krleža orcid id orcid.org/0000-0002-2554-4034 ; Department of Laboratory Diagnostics, Children’s Hospital Zagreb, Zagreb, Croatia

Full text: english pdf 100 Kb

page 307-314

downloads: 359

cite

Abstract

Introduction: The aim of this study was to examine whether the Canadian Laboratory Initiative on Paediatric Reference Intervals (CALIPER) could
be applied to Croatian children and adolescents.
Materials and methods: A total of 295 outpatient healthy children and adolescents of age 1 to 18 were selected using the direct a posteriori
sampling method. According to current guidelines, 20 samples were tested for each of a total of 51 reference intervals for ferritin, cortisol, dehydroepiandrosterone
sulfate, follicle stimulating hormone, lutein stimulating hormone, prolactin, progesterone, sex hormone binding globulin, thyroid
stimulating hormone, total testosterone, total thyroxine and total triiodothyronine. Serum samples were analysed on the Beckman Coulter DxI600
immunoassay analyser by chemiluminescence immunoassay method. A reference interval was adopted if < 10% of the results fall outside CALIPER
reference interval range. For analytes in which this criterion is not met in the first set of samples, a new set of 20 samples were collected.
Results: After the first set of measurements, 96% of all tested reference intervals were adopted for use. The additional sets of 20 reference subjects
were tested for only two reference intervals; follicle stimulating hormone for female aged 1 to 9 years, and irrespective of the gender, sex hormone
binding globulin for children aged 8 to 11 years. All results of additional samples were within the specified interval limits.
Conclusions: CALIPER reference intervals for ferritin and 11 hormones defined for Beckman Coulter DxI600 immunoassay analyser can be implemented
into the Croatian laboratories and clinical practice.

Keywords

hormone; immunoassay; paediatric; reference value

Hrčak ID:

238913

URI

https://hrcak.srce.hr/238913

Publication date:

15.6.2020.

Visits: 1.010 *