Original scientific paper
https://doi.org/10.3325/cmj.2020.61.8
DNMT3B rs1569686 and rs2424913 gene polymorphisms are associated with positive family history of preterm birth and smoking status
Anita Barišić Department of Medical Biology and Genetics, Faculty of Medicine University of Rijeka, Rijeka, Croatia
; Department of Medical Biologyand Genetics, Faculty of Medicine University of Rijeka, Rijeka, Croatia
Maja Kolak
; Department of Oncology, Clinical Hospital Center Rijeka, Rijeka,Croatia
Ana Peterlin
; Clinical Institute of Medical Genetics, University Medical Center Ljubljana, Ljubljana, Slovenia
Nataša Tul
; Department of Obstetrics and Gynecology, University MedicalCenter Ljubljana, Ljubljana,Slovenia
Milena Gašparović Krpina
; Department of Obstetrics and Gynecology, Clinical Hospital Center Rijeka, Rijeka, Croatia
Saša Ostojić
; Department of Medical Biologyand Genetics, Faculty of Medicine University of Rijeka, Rijeka, Croatia
Borut Peterlin
; Department of Obstetrics and Gynecology, Clinical Hospital Center Rijeka, Rijeka, Croatia
Nina Pereza
; Department of Biology, Faculty of Medicine, University of Rijeka,Rijeka, Croatia
Abstract
Aim To evaluate the association between spontaneous preterm birth (SPTB) and DNA methyltransferase (DNMT)1, 3A, 3B, and 3L gene polymorphisms, and their contribution to the clinical characteristics of women with SPTB and their newborns.
Methods This case-control study, conducted in 2018, enrolled 162 women with SPTB and 162 women with term delivery. DNMT1 rs2228611, DNMT3A rs1550117, DNMT3B rs1569686, DNMT3B rs2424913, and DNMT3L rs2070565 single nucleotide polymorphisms were genotyped using polymerase chain reaction and restriction fragment length polymorphism methods. The clinical characteristics included in the analysis were family history of preterm birth, maternal smoking, maternal age, gestational week at delivery, and fetal birth weight.Results DNMT gene polymorphisms were not significantly associated with SPTB. DNMT3B rs1569686 and rs2424913 minor alleles (T) were significantly more frequent in women with familial PTB than in women with non-familial PTB, increasing the odds for familial PTB 3.30 and 3.54 times under dominant genetic models. They were also significantly more frequent in women with SPTB who smoked before pregnancy, reaching the most significant association under additive genetic models (odds ratio 6.86, 95% confidence interval 2.25-20.86, P < 0.001; odds ratio 3.77, 95% confidence interval 1.36-10.52, P = 0.011, respectively).
Conclusions DNMT3B rs1569686 and rs2424913 gene polymorphisms might be associated with positive family history of PTB and smoking status.
Keywords
Hrčak ID:
240150
URI
Publication date:
15.2.2020.
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