Original scientific paper

https://doi.org/10.46419/vs.52.3.1

Effects of a probiotic containing Bacillus subtilis on the gut microflora, yolk quality and blood lipid concentrations of laying Pharaon quails

Lyudmila P. Ermakova orcid.org/0000-0003-2828-3957 ; Veterinarski fakultet, Državno Poljoprivredno Sveučilište Novosibirsk, Novosibirsk, Novosibirskaya oblast, Rusija
Grigory A. Nozdrin ; Veterinarski fakultet, Državno Poljoprivredno Sveučilište Novosibirsk, Novosibirsk, Novosibirskaya oblast, Rusija
Sergei N. Tishkov ; Veterinarski fakultet, Državno Poljoprivredno Sveučilište Novosibirsk, Novosibirsk, Novosibirskaya oblast, Rusija
Yana V. Novik ; Veterinarski fakultet, Državno Poljoprivredno Sveučilište Novosibirsk, Novosibirsk, Novosibirskaya oblast, Rusija
Nikita A. Gotovchikov ; Veterinarski fakultet, Državno Poljoprivredno Sveučilište Novosibirsk, Novosibirsk, Novosibirskaya oblast, Rusija
Irina K. Mensh ; Veterinarski fakultet, Državno Poljoprivredno Sveučilište Novosibirsk, Novosibirsk, Novosibirskaya oblast, Rusija

Abstract

The current literature is not comprehensive concerning the influence of Bacillus subtilis on lipid metabolism, egg chemical characteristics, and intestinal microbiota of Japanese quail. The aim of this study was to evaluate the effects of a Bacillus subtilis strain on yolk quality, gut bacterial populations, and total cholesterol and triglyceride concentrations in the plasma of organically farmed Pharaon quail layers. Forty-five-day-old female quails were randomly distributed into four groups of 10 birds each: a no-treatment control group and three test groups receiving powder of Bacillus subtilis DSM 32424 at a minimum rate of 1 × 106 colony forming units per gram, dissolved in drinking water at doses of 50, 75 and 100 mg per kg body weight, daily during 30 days. The use of the probiotic at doses of 50 and 100 mg resulted in significantly reduced faecal staphylococci count on day 30 (P<0.05), while no significant changes were detected in lactobacilli or coliform bacteria. This study gives the first evidence of the influence of Bacillus subtilis on acid value and carotenoid levels in egg yolks laid by quails. Carotenoids were significantly elevated in the 75 mg group on day 30 (P<0.05), but significantly decreased in the 50 and 100 mg groups (P<0.05). Acidity, triglyceride and total cholesterol concentrations in quail serum were not affected significantly by the treatment throughout the study, though their values were reduced. The inclusion of Bacillus subtilis DSM 32424 in drinking water for laying Pharaon quails inhibited faecal staphylococci proliferation and enhanced yolk carotenoid content. Therefore, it can be proposed that including this probiotic in laying quail diets may have beneficial outcomes for both layers and the second generation.

Keywords

quail; Bacillus subtilis; gut microflora; triglyceride; total cholesterol; yolk

Hrčak ID:

241130

URI

https://hrcak.srce.hr/241130

Publication date:

16.12.2021.

Article data in other languages: croatian

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