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Veterinary Archives, Vol. 78 No. 1, 2008.

Original scientific paper

Development of single serum dilution ELISA for detection of infectious bursal disease virus antibodies

Pachaikani Ramadass ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India
Manoharan Parthiban ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India
Venkatachalam Thiagarajan ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India
Meenakshmisundram Chandrasekar ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India
Mahalinga Nainar Vidhya ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India
Gopal Dhinakar Raj ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India

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Abstract

Determination of antibody titres of sera collected from poultry flock to diagnose IBDV infected chickens by developing single serum working dilution ELISA (SSD-ELISA) was made. The indirect ELISA standard procedure was adopted with standard positive and negative serum controls. The standard IBDV antigen was prepared using the Tamil Nadu isolate of IBDV. The relative sensitivity and specificity of a single serum dilution ELISA test in qualitative comparison with the commercial kit were 82% and 100% respectively and the accuracy was 82.1%. This single serum dilution assay gave reproducible results and allowed considerable savings on the time and cost of reagents compared with indirect ELISA. Based on these results a SDS-ELISA kits have been developed in this study to replace the commercial kit. The IBDV antigen coated ELISA plates can be stored under refrigeration and the test can be performed rapidly under field conditions by trained personnel.

Keywords

serum; ELISA; infectious bursal disease; antibody titer

Hrčak ID:

24245

URI

https://hrcak.srce.hr/24245

Publication date:

20.2.2008.

Article data in other languages: croatian

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