Original scientific paper

https://doi.org/10.20302/NC.2020.29.8

Springs: DNA barcoding of caddisflies (Insecta, Trichoptera) in Croatia with notes on taxonomy and conservation biology

Mladen Kučinić ; Department of Biology (*Laboratory for Entomology), Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
Anđela Ćukušić ; Ministry of Environment and Energy, Radnička cesta 80/7, 10000 Zagreb, Croatia
Sanja Žalac ; ZSC „Dr. Ivo Pevalek“, Plitvice Lakes National Park, Josipa Jovića 19, 53231 Plitvička jezera, Croatia
Antun Delić ; Nikole Šubića Zrinskog 3, 43290 Grubišno Polje, Croatia
Darko Cerjanec ; Barilović Primary school, Barilović 96, 47252 Barilović, Croatia
Martina Podnar ; Croatian Natural History Museum, Demetrova 1, 10000 Zagreb, Croatia
Renata Ćuk ; Hrvatske vode, Central Water Management Laboratory, Ulica grada Vukovara 220, 10000 Zagreb, Croatia
Ivan Vučković ; Elektroprojekt d.d., Civil and Architectural Engineering Department, Water Resources, Nature and Environmental Protection, Alexandera von Humboldta 4, 10000 Zagreb, Croatia
Ana Previšić ; Department of Biology (*Laboratory for Entomology), Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
Marijana Vuković ; Croatian Natural History Museum, Demetrova 1, 10000 Zagreb, Croatia
Svjetlana Stanić Koštroman ; Department of Biology, Faculty of Science and Education, University of Mostar, Matice hrvatske, 88000 Mostar, Bosnia and Herzegovina
Višnja Bukvić ; University of Hercegovina, Blajburških žrtava 100, 88000 Mostar, Bosnia and Herzegovina
Ana Šalinović ; Postelska ulica 10, 2000 Maribor, Slovenia
Mladen Plantak ; Elektroprojekt d.d., Civil and Architectural Engineering Department, Water Resources, Nature and Environmental Protection, Alexandera von Humboldta 4, 10000 Zagreb, Croatia

Abstract

The paper provides the results of DNA barcoding based on the cytochrome c oxidase subunit 1 mitochondrial gene (mtCOI) of 110 Trichoptera specimens collected in 36 springs in the PannonianPeripannonian, central mountainous and Mediterranean part of Croatia. We barcoded 70 species from 32 genera and 15 families. The data obtained show interesting faunistic and taxonomic results, for, for example, the species Rhyacophila cabrankensis, R. balcanica, Crunoecia kempnyi, Allogmaus auricollis and emphasize the need for further faunistic research into springs, in their role as habitats with a specific and very interesting fauna. The mtCOI DNA barcoding should be included in such research, because it would enable better presentation of the results, especially regarding biodiversity, taxonomy, phylogeny and conservation biology, not just as a segment of a local but also of a global process of understanding biodiversity in a different way. The results of this study show a global need for the protection of springs, because they are specific not only as habitats, but also as localities with an interesting fauna and often endemic species of very limited distribution (for example Rhyacophila cabrankensis).

Keywords

upper stream reaches; caddisflies; biodiversity; molecular methods; Rhyacophila cabrakenensis

Hrčak ID:

245493

URI

https://hrcak.srce.hr/245493

Publication date:

30.10.2020.

Article data in other languages: croatian

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