Veterinary Archives, Vol. 77 No. 2, 2007.
Original scientific paper
Immunoprophylaxis of rabbits against Rhipicephalus haemaphysaloides ticks using immunoaffinity purified 35 kDa midgut antigen
Kaveri Theerthagiri Kavitha
; Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Bhaskaran Ravi Latha
; Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Gopal Dhinakar Raj
; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Lalitha John
; Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Abstract
A 35 kDa midgut polypeptide of Rhipicephalus haemaphysaloides was isolated for the first time by immunoaffinity chromatography using immunoglobulin ligands from rabbits immunized with 35 kDa midgut polypeptide. An experimental immunization study was conducted on 12 New Zealand white rabbits using affinity purified 35 kDa midgut antigen (Aff - RhGM 35 kDa Ag) with Freund’s adjuvant. The humoral immune response was evaluated by ELISA. After tick challenge, reduction in the number, mass and oviposition capacity of engorged females was observed in the tick population that had fed on immunized animals. The results indicated a high efficacy of 83.3%, demonstrating the efficiency of the immune response elicited by 35 kDa midgut antigen to control the tick Rhipicephalus haemaphysaloides.
Keywords
Rhipicephalus haemaphysaloides; 35 kDa midgut antigen; immunoaffinity chromatography; immunization; rabbits
Hrčak ID:
25057
URI
Publication date:
20.4.2007.
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