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Veterinarska stanica, Vol. 52 No. 4, 2021.

Review article

https://doi.org/10.46419/vs.52.4.9

CRISPR/Cas9 uses: a review

Nora Mimoune orcid id orcid.org/0000-0002-0900-3908 ; National High School of Veterinary Medicine, Algiers, Algeria; National Institute of Veterinary Sciences, Laboratory of Biotechnology Related to Animal Reproduction (LBRA), University Saad Dahleb, Blida, Algeria
Oumayma Benadjel ; National Institute of Veterinary Sciences, Laboratory of Biotechnology Related to Animal Reproduction (LBRA), University Saad Dahleb, Blida, Algeria
Ratiba Baazizi ; National Institute of Veterinary Sciences, Laboratory of Biotechnology Related to Animal Reproduction (LBRA), University Saad Dahleb, Blida, Algeria
Djamel Kelef ; National Institute of Veterinary Sciences, Laboratory of Biotechnology Related to Animal Reproduction (LBRA), University Saad Dahleb, Blida, Algeria
Rachid Kaidi ; Laboratory of Biotechnology Related to Animal Reproduction (LBRA), University Saad Dahleb, Blida, Algeria

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Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR- associated system (Cas) is a system that provides immunity to most prokaryotic organisms against viral attacks and other foreign bodies. CRISPR systems consist of a scissor-like protein called Cas9 and a genetic GPS guide “The guide RNA”. However, researchers have reoriented and repurposed the primordial immune system to precisely manipulate genomes in most organisms by introducing DNA double-strand breaks at specific genome locations to introduce specific
DNA modifications. More applications of CRISPR have arisen since its discovery, from disabling parasites to correcting mutations and improving crop yields. This review was conceived as a guide to CRISPR technology, from its discovery to the latest breakthroughs. It is hoped that this study will provide a general-based view for this life changing technology, inspiring scientists to go further with CRISPR in the sake of a better life.

Keywords

CRISPR/Cas9; DNA; RNA; genome engineering; bacteria; virus

Hrčak ID:

252031

URI

https://hrcak.srce.hr/252031

Publication date:

1.2.2021.

Article data in other languages: croatian

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