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Croatian Medical Journal, Vol. 61 No. 3, 2020.

Original scientific paper

https://doi.org/10.3325/cmj.2020.61.107

A mathematical model reveals sex-specific changes in glucose and insulin tolerance during rat puberty and maturation

Marta Balog orcid id orcid.org/0000-0002-7842-4781 ; Department of Medical Biology and Genetics, Faculty of Medicine Osijek, J. J. Strossmayer University of Osijek, Osijek, Croatia
Vedrana Ivić orcid id orcid.org/0000-0002-8185-1960 ; Department of Medical Biology and Genetics, Faculty of Medicine Osijek, J. J. Strossmayer University of Osijek, Osijek, Croatia
Rudolf Scitovski ; Department of Mathematics, J. J. Strossmayer University of Osijek, Osijek, Croatia
Irena Labak ; Department of Biology, J. J. Strossmayer University of Osijek,Osijek, Croatia
Kálmán Ferenc Szűcs orcid id orcid.org/0000-0002-5878-4691 ; Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary
Robert Gaspar orcid id orcid.org/0000-0002-1571-7579 ; Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary
Sándor G. Vári ; Cedars-Sinai Medical Center, International Research and Innovation in Medicine Program,Los Angeles, CA, United States
Marija Heffer orcid id orcid.org/0000-0001-6770-7359 ; Department of Medical Biology and Genetics, Faculty of Medicine Osijek, J. J. Strossmayer University of Osijek, Osijek, Croatia

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Abstract

Aim To evaluate the effects of maturation and sex on glu-
cose metabolism during glucose tolerance (GTT) and in-
sulin tolerance tests (ITT) in young and adult male and
female rats by using two different approaches – the con-
ventional, which uses area under the curve and glucose
curve, and mathematical modeling that identifies param-
eters necessary for determining the function that models
glucose metabolism.
Methods Male and female rats at 3.5 and 12 months of
age underwent standard GTT and ITT after overnight fast-
ing. The parameters were identified by using Mathemat-
ica-module NonlinearModelFit [ ] for experimentally ob-
tained data.
Results When data were statistically analyzed, both sexes
and age groups had similar glucose and insulin tolerance.
In the mathematical model of GTT, parameters describing
the rate of glucose concentration increase G’(0) and de-
crease G’I multiplied with maturation, with a concomitant
decrease in the time point (tmax, tI) of reaching maximum
and minimum glucose concentration (Gmax, G0). The math-
ematical model of ITT for males was independent of age,
unlike of that for females, which had increased G’(0) and
G’I, and more quickly recovered from hypoglycemia after
maturation.
Conclusion The mathematical model revealed female
susceptibility to large glucose excursions, which are better
reflected by ITT in young animals and by GTT in adults.

Keywords

Hrčak ID:

253143

URI

https://hrcak.srce.hr/253143

Publication date:

14.6.2020.

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