Original scientific paper

https://doi.org/10.2478/acph-2022-0001

UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca

RAFEEF K. MASSADEH ; Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan
TAMAM EL-ELIMAT ; Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan
MOHAMMAD AL-GHARAIBEH ; Department of Plant Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid 22110, Jordan
KHALED TAWAHA ; Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman 11942, Jordan
FERAS Q. ALALI ; College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar

Abstract

The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild Tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g–1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydrodistillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.

Keywords

Nicotiana glauca; tobacco tree; UPLC-HRESI-MS; GC-MS; anabasine; nicotine; volatile oil; methanolic extract

Hrčak ID:

253981

URI

https://hrcak.srce.hr/253981

Publication date:

31.3.2022.

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