Original scientific paper

https://doi.org/10.24099/vet.arhiv.1024

In vitro anthelmintic efficacy of Citrullus colocynthis (L.) Schrad on Haemonchus contortus

Tauseef Rehman ; University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan
Khalid J. Iqbal ; Department of Zoology, The Islamia University of Bahawalpur, Pakistan
Azra Anwer ; Department of Zoology, The Islamia University of Bahawalpur, Pakistan
Rao Z. Abbas orcid.org/0000-0001-7344-6212 ; Department of Parasitology, University of Agriculture, Faisalabad, Pakistan
Wasim Babar ; Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan
Ahmad Ali ; Department of Zoology, The Islamia University of Bahawalpur, Pakistan
Muhammad K. Khan ; Department of Parasitology, University of Agriculture, Faisalabad, Pakistan
Arshad Javid ; University of Veterinary and Animal Sciences, Lahore, Pakistan
Noor Khan ; University of Veterinary and Animal Sciences, Lahore, Pakistan
Hafiz M. Ali ; University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan
Faisal Shahzad ; University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan
Muhammad A. Zaman ; Department of Pathobiology, College of Veterinary and Animal Sciences, Jhang, Pakistan
Arbab Sikandar ; Department of Anatomy, College of Veterinary and Animal Sciences, Jhang, Pakistan

Abstract

Ethno-veterinary medicinal studies associated with traditional uses of the flora of the Cholistan desert have shown that fruits of Citrullus colocynthis are used for the treatment of helminth infections. The present research was designed to evaluate the anthelmintic efficacy of C. colocynthis against H. contortus. The in vitro anthelmintic effects of aqueous-methanol and ethyl acetate fruit extracts of C. colocynthis against H. contortus were determined through egg hatch and adult motility assays. The effect of four serial dilutions of 25 mg/mL of each extract compared to levamisol (0.55 mg/mL) and oxfendazole (three serial dilutions of 25 μg/mL) were studied. Both ethyl acetate and aqueous-methanol extracts paralyzed all adult worms 4h and 8h post-exposure at a dose of 25 mg/mL each. In the egg hatch assay, about 83.67% and 80.67% of H. contortus eggs failed to hatch with the same dose (i.e. 25 mg/mL) of ethyl acetate and CAME extracts, respectively. The results of the present study strongly support fruit extracts of C. colocynthis as a promising alternative to synthetic drugs against H. contortus. These findings will lead to further in vivo studies to investigate the bio-availability of the active ingredients of the plant and the minimum non-lethal concentration required for treatment of haemonchosis in livestock. The anthelmintic effects of C. colocynthis might be attributed to the presence of phenolic acids.

Keywords

anthelmintic efficacy; C. colocynthis; H. contortus; egg hatch assay; adult motility assay

Hrčak ID:

260232

URI

https://hrcak.srce.hr/260232

Publication date:

9.7.2021.

Article data in other languages: croatian

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