Original scientific paper

https://doi.org/10.2478/acph-2022-0027

Potential anti-aging effects of probiotic-derived conditioned media on human skin cells

YOO KYUNG HONG ; Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea
SUNGKWAN AN ; Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea
YUN HEE LEE ; Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea
SEUNG AH YANG ; Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea
YOO KYUNG YOON ; Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea
JOONIL LEE ; Research and Development Center, Cell Biotech, Co., Ltd., Gimpo 10003, Republic of Korea
GWASOO LEE ; Research and Development Center, Cell Biotech, Co., Ltd., Gimpo 10003, Republic of Korea
MYUNG JUN CHUNG ; Research and Development Center, Cell Biotech, Co., Ltd., Gimpo 10003, Republic of Korea
SEUNGHEE BAE ; Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea

Abstract

In this study, the protective functions of bacteria-free conditioned media from Bifidobacterium and Lactobacillus species against ultraviolet radiation-induced skin ageing and associated cellular damage were investigated. The effects of ultraviolet radiation-induced reactive oxygen species production were suppressed by all conditioned media; particularly, the loss of cell viability and downregulation of collagen gene expression were significantly reversed by the conditioned media from B. longum and B. lactis. Further examination of potential anti-pigmentation effects revealed that the B. lactis-derived conditioned media significantly inhibited tyrosinase activity and alpha-melanocyte-stimulating hormone-induced melanin production in human epidermal melanocytes. Further, the conditioned media suppressed the phosphorylation of extracellular signal-related kinase, which functions as an upstream regulator of melanogenesis. Therefore, B. lactis-derived conditioned media can potentially protect against cellular damage involved in skin-ageing processes.

Keywords

anti-aging; skin cells; probiotics; conditioned media; protection; anti-pigmentation

Hrčak ID:

268191

URI

https://hrcak.srce.hr/268191

Publication date:

30.9.2022.

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