Original scientific paper
Dose- and Mode-Dependent Effect of Halogen Dental Curing Blue Light on the V79-Cell Line
Silvana Jukić Krmek
Ivan~ica Trošić
Ivan Pavičić
Jadranka Keros
Paris Simeon
Ana Malčić
Goranka Prpić-Mehičić
Abstract
The aim of this study was to evaluate the time and dose dependent effect of halogen light from dental curing unit on
the cell viability, colony-forming ability and proliferation of the V79 cell culture. The investigation included the medium
mode (M), exponential (E) and standard (S) illumination mode for 20, 40 and 80 seconds. The viability was determined
using the trypan blue exclusion test. Colony forming ability was assessed by colony count on post-exposure day 7. Cell
proliferation was determined by cell counts during five post-exposure days. The viability of cells was not affected by blue
light in view of exposure time and modes. Colony forming ability in treated cells was slightly, but not significantly lower
than in control cells. Cell proliferation was lower in cells exposed to the M mode for 80 s on post-exposure day 3 and 4
(p<0.05). On the same post-exposure days, the proliferation of cells exposed to modes E and S, showed a significant inhibition
after 20, 40 and 80 s of exposure (p<0.05). Disrupted cellular functionality and no significant decrease in colony
forming ability of V79 cells in addition to time- and dose dependent significant inhibition of cell proliferation might be
ascribed to the photocuring blue light activity and/or changes in temperature during the course of experiment in vitro.
Keywords
halogen lamp; photo-polymerization; cell culture; viability; colony forming ability; cell proliferation
Hrčak ID:
27030
URI
Publication date:
2.9.2008.
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