Original scientific paper
Biological Bases of Dentin Hybridization
Davor Brajdić
Ozren Mika Krznarić
Zoran Azinović
Darko Macan
Marijan Baranović
Abstract
The aims of this study were threefold: (1) to characterize and quantify the number, diameter and surface area of exposed
dentinal tubules on the cross section of the human coronal dentin; (2) to determine if any such differences in these
properties arise in relation to the distance from the dentinoenamel junction; and (3) to evaluate whether such differences
can influence dentin hybridization. To accomplish these aims, scanning electron microscopy comparative observation
was carried out on 60 prepared human premolars, which were divided into three groups of 20 samples each. The three
sample groups were cut as follows: (1) in the central fissure region, one millimeter from the enamel-dentine junction; (2)
halfway between the enamel-dentine junction and the pulp; and (3) one millimeter from the roof of the pulp chamber.
Using one-way analysis of variance (one-way ANOVA) and a regression linear model, the data enumerated below were
obtained. First, the mean number of the tubule openings was 19600/mm2 on the first level, 32400/mm2 on the second and
42300/mm2 on the third. The mean tubule diameter on the first level was 0.67 mm, 1.52 mm on the second and 2.58 mm on
the third. Finally, exposed tubules on the first level occupied 2.79% of of total dentinal surface area, 23.90% on the second,
and 87.78% on the third level. Therefore, significant statistical differences (p<0.01) between all three groups of the
specimens for all three properties were observed, as well as positive correlation between the dentin depth and each of these
properties. This indicates that the dentin structural variety, which ultimately determines adhesion to dentine, involves a
complex interaction between biological material (dentin) and the particular adhesion system applied.
Keywords
dentin; dentinal tubules; dentin adhesives; dentin hybridization; hybrid layer; scanning electron microsopy
Hrčak ID:
27045
URI
Publication date:
2.9.2008.
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