Original scientific paper
Toxicological Assessment of P-9801091 Plant Mixture Extract after Chronic Administration in CBA/HZg Mice – A Biochemical and Histological Study
Roberta Petlevski
Mirko Hadžija
Milivoj Slijepčević
Dubravka Juretić
Abstract
Acute, subchronic and chronic effects of the P-9801091 plant mixture extract at a dose of 20 mg/kg body mass were assessed
in serum of healthy CBA/HZg mice at 24 hours, 7 days, 3 months and 6 months of treatment (experimental group),
and compared with the values obtained in the control group of untreated healthy CBA/HZg mice. The P-9801091 plant
mixture extract is an antihyperglycemic preparation containing Myrtilli folium (Vaccinium myrtillus L.), Taraxaci radix
(Taraxacum officinale Web.), Cichorii radix (Cichorium intybus L.), Juniperi fructus (Juniperus communis L.), Centaurii
herba (Centaurium umbellatum Gilib.), Phaseoli fructus sine semine (Phaseolus vulgaris L.), Millefolii herba (Achillea
millefolium L.), Mori folium (Morus nigra L.), Valerianae radix (Valeriana officinalis L.) and Urticae herba et radix
(Urtica dioica L). Toxic effect of the P-9801091 plant mixture extract was assessed by the following biochemical parameters:
urea, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cholesterol. Also, histopathological
examination of the kidneys, liver, spleen, pancreas, testes and lungs was performed. Results of biochemical
testing performed at specified time points generally showed no statistically significant differences from control values,
with the only exception of the catalytic concentration of AST in the experimental group measured on day 7, which was
significantly increased as compared with the control group (p<0.05). Pathohistological examination including characteristic
organ and tissue structure, and parenchyma relationship to the adjacent blood vessels and connective tissue in
the examined organs revealed no major pathologic changes.
Keywords
CBA mice; chronic toxicity; aminotransferase; urea and creatinine; histopathology; plant extract
Hrčak ID:
27180
URI
Publication date:
7.7.2008.
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