Original scientific paper
Distribution of Human Papillomavirus Types in Different Histological Subtypes of Cervical Adenocarcinoma
Ita Hadžisejdić
Maja Krašević
Herman Haller
Blaženka Grahovac
Abstract
Little information is available regarding distribution of HPV types in different histological subtypes of adenocarcinoma
(AC). Thus, in this study we examined the frequency of high-risk (hr) HPV types in AC, adenocarcinoma in situ
(AIS) and adenosquamous carcinoma (ADSQ). A total of 102 cases of primary cervical adenocarcinoma (26 AIS and 76
invasive AC) obtained from pathology files from 1995–2006 were histologically subtyped. Our results demonstrated that
endocervical type occupied the major subtype of AC (22/66) followed by ADSQ (17/66) where as in the group of AIS
endocervical type (12/23) was followed by intestinal type of AIS (7/23). Successful DNA extraction was obtained in 89
samples; 81 out of 89 (91.0%) tested positive for HPV DNA. The prevalence of HPV DNA in AIS, AC and ADSQ was
91.3% (21/23), 90.9% (60/66) and 94.1% (16/17), respectively. We found HPV 18 type to be the most predominant type in
AIS (11/21) and AC (17/60) followed by HPV of undeternmined type in AIS (3/21) and HPV 16 in AC (9/60) as the sole viral
type. HPV 18 was most frequently detected type in all histological subtypes of AIS and AC. We have detected HPV
DNA in all 5 samples of clear cell carcinoma (CCC), although other studies have reported a highly variable prevalence of
HPV DNA in CCC. The most prevalent HPV type in ADSQ was HPV-16 followed by HPV 33 as single type. The observed
overall predominance of HPV 18 in AIS (
2= 6.109, p£ 0.025) and AC (
2 = 8.927, p£0.01) as well as of HPV 16 in ADSQ
(
2 = 10.164, p £ 0.01) was statistically significant. Our data revealed statistically significant predominance of single
hrHPV infections in AIS (16/21;
2 = 11.523, p £ 0.001) and AC (37/60;
2 = 6.533, p £ 0.025) whereas multiple hrHPV
infections were more abundant in AC comparing to AIS (23/81and 5/81, respectively;
2 = 13.989, p £ 0.001).
Keywords
cervical adenocarcinoma; adenocarcinoma in situ; histological subtypes; HPV prevalence frequency and typing
Hrčak ID:
27449
URI
Publication date:
13.4.2007.
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