Original scientific paper
https://doi.org/10.3325/cmj.2021.62.6 8
Distribution of human papillomavirus genotypes in women with high-grade cervical intraepithelial lesions and cervical carcinoma and analysis of human papillomavirus-16 genomic variants
Magdalena Karadža
; Department of Gynecologic Oncology, Clinic for Gynecology and Obstetrics, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Snjezana Židovec Lepej
orcid.org/0000-0001-9852-5806
; Dr. Fran Mihaljević University Hospital for Infectious Diseases, Zagreb, Croatia
Ana Planinić
; Dr. Fran Mihaljević University Hospital for Infectious Diseases, Zagreb, Croatia
Ivana Grgić
; Dr. Fran Mihaljević University Hospital for Infectious Diseases, Zagreb, Croatia
Ante Ćorušić
; Department of Gynecologic Oncology, Clinic for Gynecology and Obstetrics, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Pavao Planinić
; Department of Gynecologic Oncology, Clinic for Gynecology and Obstetrics, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Mario Ćorić
; Department of Gynecologic Oncology, Clinic for Gynecology and Obstetrics, Clinical Hospital Centre Zagreb, Zagreb, Croatia
Lea Hošnjak
; Institute of Immunology and Microbiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Kristina Fujs Komloš
; Institute of Immunology and Microbiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Mario Poljak
; Institute of Immunology and Microbiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Adriana Vince
; University of Zagreb School of Medicine, Zagreb, Croatia
Abstract
Aim To analyze the distribution of high-risk human papil
-
lomavirus (HR-HPV) genotypes and the diversity of HPV16 genomic variants in Croatian women with high-grade
squamous intraepithelial lesions (HSIL) and cervical carci
-
noma.
Methods Tissue biopsy specimens were obtained from
324 women with histopathologically confirmed HSIL or
cervical carcinoma, 5 women with low-grade SIL, and 49
women with negative histopathology. HR-HPV DNA was
detected with Ampliquality HPV-type nucleic-acid hybrid
-
ization assay, which identifies 29 different HPV genotypes.
HPV-16 genomic variants were analyzed by an in-house se
-
quencing.
Results The most common HPV type in women with HSIL
was HPV-16, detected in 127/219 (57.9%) specimens. HPV16 was also the dominant type in squamous cell cervical
carcinoma (46/69 or 66.7%) and in adenocarcinoma (18/36
or 50.0%). Out of 378 patients, 360 had HR-HPV (282 sin
-
gle infections and 79 multiple infections), 3 (0.8%) patients
had low-risk HPV, and 15 (4%) tested negative. HPV-16 vari
-
ants were determined in 130 HPV-16 positive specimens,
including 74 HSIL and 46 carcinoma specimens. In HSIL
specimens, 41 distinct variants were found, 98.6% belong
-
ing to the European branch and 1.4% belonging to the African branch. In cervical carcinoma specimens, 95% isolates
grouped in 41 variants belonging to the European branch,
one isolate (2.5%) belonged to the North American, and
one (2.5%) to the Asian-American branch.
Conclusion HPV-16, mainly belonging to the European
branch, was the most frequent HPV genotype in women
from Croatia with histologically confirmed HSIL and cervi
-
cal cancer.
Keywords
Hrčak ID:
278033
URI
Publication date:
25.2.2021.
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