Original scientific paper
https://doi.org/10.3325/cmj.2021.62.154
B regulatory cells and monocyte subpopulations in patients with chronic graft-vshost diseas
Antonija Babić
; Department of Laboratory Diagnostics, University Hospital Centre Zagreb, Zagreb, Croatia
Lejla Kurić
; Department of Laboratory Diagnostics, University Hospital Centre Zagreb, Zagreb, Croatia
Ana Zelić Kerep
; Department of Internal Medicine, Division of Hematology, University Hospital Centre Zagreb, Zagreb, Croatia
Lana Desnica
; Department of Internal Medicine, Division of Hematology, University Hospital Centre Zagreb, Zagreb, Croatia
Antonela Lelas
; Department of Internal Medicine, Division of Hematology, University Hospital Centre Zagreb, Zagreb, Croatia
Milan Milošević
; Department of Environmental Health, Occupational and Sports Medicine, Andrija Štampar School of Public Health, University of Zagreb School of Medicine, Zagreb, Croatia
Ranka Serventi-Seiwerth
; Department of Internal Medicine, Division of Hematology, University Hospital Centre Zagreb, Zagreb, Croatia
Nadira Duraković
; Department of Internal Medicine, Division of Hematology, University Hospital Center Zagreb and University of Zagreb School of Medicine, Zagreb, Croatia
Zinaida Peric
; Department of Internal Medicine, Division of Hematology, University Hospital Center Zagreb and University of Zagreb School of Medicine, Zagreb, Croatia
Marinka Mravak Stipetić
; University Hospital Center Zagreb and University of Zagreb School of Dental Medicine
Ervina Bilic
; Department of Neurology, University Hospital Center Zagreb and University of Zagreb School of Medicine, Zagreb, Croatia
Romana Čeović
; Department of Dermatology, University Hospital Center Zagreb, University of Zagreb School of Medicine, Zagreb, Croatia
Marko Barešić
; Department of Internal Medicine, Division of Clinical Immunology and Rheumatology University Hospital Center Zagreb, University of Zagreb School of Medicine, Zagreb, Croatia
Tamara Vukić
; Department of Rehabilitation and Orthopaedic Aids, University Hospital Center Zagreb, Zagreb, Croatia
Dina Ljubas Kelečić
; Department of Internal Medicine, Section for Clinical Nutrition, University Hospital Center Zagreb, Zagreb, Croatia
Sanja Mazić
; Department of Transfusion Medicine and Transplantation Biology, University Hospital Center Zagreb, Zagreb, Croatia
Ines Bojanić
; Department of Transfusion Medicine and Transplantation Biology, University Hospital Center Zagreb, Zagreb, Croatia
Ana Hećimović
; Department of Pulmonology, University Hospital Center Zagreb, Zagreb, Croatia
Ernest Bilic
; Department of Pediatrics, University Hospital Center Zagreb and University of Zagreb School of Medicine, Zagreb, Croatia
Renata Zadro
; Department of Pediatrics, University Hospital Center Zagreb and University of Zagreb School of Medicine, Zagreb, Croatia
Radovan Vrhovac
; Department of Internal Medicine, Division of Hematology, University Hospital Center Zagreb and University of Zagreb School of Medicine, Zagreb, Croatia
Steven Z. Pavletic
; National Cancer Institute, Center for Cancer Research, National Institutes of Health, Bethesda, MD, USA
Drago Batinić
; Department of Laboratory Diagnostics, University Hospital Center Zagreb and University of Zagreb School of Medicine, Zagreb, Croatia
Dražen Pulanić
; Department of Internal Medicine, Division of Hematology, University Hospital Center Zagreb and University of Zagreb School of Medicine, Zagreb, Croatia
Abstract
Aim To assess the correlations of B regulatory cells (Bregs)
and monocyte subsets in peripheral blood with the National Institutes of Health (NIH)-consensus-defined clinical
manifestations of chronic graft-vs-host disease (cGvHD), in
an attempt to establish their role as cellular biomarkers.
Methods This multidisciplinary prospective study enrolled
adult cGVHD patients treated in the University Hospital
Center Zagreb and University of Zagreb School of Medicine. Immunophenotypic subpopulations of CD24highCD38high Bregs (CD27-, CD27+, and total) and monocyte
(classical, intermediate, and non-classical) counts were
correlated with demographic, transplant, and cGVHD-related data. Bivariate correlation analysis was performed to
evaluate the correlations between Bregs and monocytes
subsets and cGVHD organ involvement, as well as cGVHD
severity and immunosuppression intensity.
Results Twenty-two adult patients (54.5% female) with
cGVHD were enrolled. The median (range) age was 44.5
years (24-65). All patients were transplanted for hematologic malignancies and 40.9% had severe NIH cGVHD
global score. The median time from cGVHD diagnosis to
the analysis was 16.6 months (0-176). The organ most frequently affected with cGVHD were the eyes (68.2%), skin
(45.5%), lungs (45.5%), and liver (40.9%). Lower total and
CD27-Bregs counts were correlated with worse cGVHD
severity, higher immunosuppression intensity, and lung
cGVHD, in terms of cell count, but also with skin cGVHD,
in terms of percentages. Patients with liver and joint/fascia cGVHD had a lower percentage of non-classical monocytes and patients with more severe global NIH score had
a higher classical monocytes count.
Conclusion Different organs affected by cGVHD are differently associated with different subpopulations of Bregs
and monocytes.
Keywords
Hrčak ID:
278125
URI
Publication date:
29.4.2021.
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