Original scientific paper
https://doi.org/10.26332/seemedj.v6i2.256
Fractionation of Vipera berus berus Snake Venom and Detection of Bioactive Compounds Targeted to Blood Coagulation System
Eldar Iskandarov
; Palladin Institute of biochemistry, NAS of Ukraine; Biology And Medicine Institute Science Educational Center Of Taras Shevchenko National University Of Kyiv, Ukraine
Volodymyr Gryshchuk
; Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine
Oleh Platonov
; Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine
Yevhenii Kucheriavyi
; Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine
Oleksandr Slominskyi
; Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine
Yevhenii Stohnii
; Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine
Volodymyr Vartanov
; Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine
Volodymyr Chernyshenko
; Palladin Institute of Biochemistry, NAS of Ukraine, Ukraine
Abstract
Introduction. The performed research focused on a search for new biologically active compounds acting on blood coagulation system proteins and cells. To achieve this goal, we fractionated Vipera berus berus snake venom and studied the action of the separated fractions on human blood plasma, fibrinogen, platelets or red cells.
Methods. Crude venom was fractionated using ion-exchange chromatography. Protein composition of fractions was studied using SDS-PAGE. The ability of fractions to prolong or initiate blood plasma clotting was studied using the prothrombin time test with thromboplastin. Fibrinogen-specific proteases were detected using enzyme-electrophoresis. Action on red cells was estimated using the hemolysis test. Aggregometry was used for the detection of action on platelets. All experiments in this study were performed in vitro.
Results. We obtained fractions containing phospholipase and a protease that is able to hydrolyze fibrinogen, leading to the loss of its ability to polymerize and to maintain platelet aggregation.
Conclusion. Further purification and study of these components can be a promising research direction for biotechnological as well as for biomedical use.
Keywords
snake venoms; blood coagulation; fibrinogen; hemolysis; platelet aggregation
Hrčak ID:
286290
URI
Publication date:
28.11.2022.
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