Original scientific paper

https://doi.org/10.5562/cca3994

Diterpenes and Phenolic Compounds from Salvia brachyodon Vandas

Franz Bucar orcid.org/0000-0002-7417-616X ; Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Beethovenstraße 8, Graz, Austria
Božena Mitić ; Division of Botany, Department of Biology, University of Zagreb Faculty of Science, Marulićev trg 9a, Zagreb, Croatia
Dario Hruševar ; Division of Botany, Department of Biology, University of Zagreb Faculty of Science, Marulićev trg 9a, Zagreb, Croatia
Olaf Kunert ; Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemsitry, University of Graz, Universitätsplatz 1, Graz, Austria
Kerstin Binder ; Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Beethovenstraße 8, Graz, Austria
Sandra Ochensberger ; Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Beethovenstraße 8, Graz, Austria
Maja Friščić ; Department of Pharmaceutical Botany, University of Zagreb, Faculty of Pharmacy and Biochemistry, Schrottova 39, Zagreb, Croatia
Sandra Jurić ; Department of Organic Chemistry, University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, Zagreb, Croatia
Tihana Marić ; Department of Pharmaceutical Botany, University of Zagreb, Faculty of Pharmacy and Biochemistry, Schrottova 39, Zagreb, Croatia
Željan Maleš orcid.org/0000-0003-1034-2525 ; Department of Pharmaceutical Botany, University of Zagreb, Faculty of Pharmacy and Biochemistry, Schrottova 39, Zagreb, Croatia

Abstract

Salvia brachyodon, the short-tooth sage, is one of the rarest plant species and endemic in the Adriatic area of the Balkan Peninsula. As aside from its essential oil, only limited information on its phytochemical composition is known, a more detailed study of the leaves was undertaken. From its leaves two diterpenes, agastanol (2), and a new natural compound 1, i.e., 3-methyl-4-methylen-11,12,14-trihydroxy-8,11,13-abietatrien-7-one, were isolated and identified by NMR spectroscopy and mass spectrometry. In addition, caffeic acid, isoquercitrin, luteolin 7-O-glucoside and rosmarinic acid were identfied by comparison with reference compounds. The fraction containg the diterpenes as well as the isolated compound 1 showed significant antimycobacterial activity against Mycobacterium smegmatis. The diterpenes of S. brachyodon represent promising antimycobacterial substances for further evaluation. Due to the endangered nature of the plant, the wide use of S. brachyodon and its bioactive compounds could be achieved by growing the plants in culture.

Keywords

Salvia brachyodon Vandas; diterpenes; phenolic compounds; antimycobacterial activity; LC-PDA-ESI-MS; NMR

Hrčak ID:

305850

URI

https://hrcak.srce.hr/305850

Publication date:

6.7.2023.

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