Veterinary Archives, Vol. 93 No. 3, 2023.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.1698
Chromosomal aberrations induced by Mitomycin C in canine lymphocytes
Martina Noel Caliri
; National Council of Scientific and Technical Research (CONICET), Argentina; Laboratory of Genetics, Environment and Reproduction, Juan Augustin Maza University, Guaymallen, Mendoza, Argentina
Daniela Marisol Ferré
; National Council of Scientific and Technical Research (CONICET), Argentina; Laboratory of Genetics, Environment and Reproduction, Juan Augustin Maza University, Guaymallen, Mendoza, Argentina
Valeria Ledda
; University of Anconcagua, Mendoza, Argentina
Nora Bibiana Maria Gorla
; National Council of Scientific and Technical Research (CONICET), Argentina; Laboratory of Genetics, Environment and Reproduction, Juan Augustin Maza University, Guaymallen, Mendoza, Argentina
Abstract
Chromosomal aberrations comprise an abnormal number of chromosomes as well as changes in the structure of the chromosomes. Aberrations in the structure of chromosomes, such as gaps and breaks in chromatids and chromosomes, acentric fragments, telomeric associations, deletions, early chromatid-separation and large scale effects, such as pulverized metaphases, and sticky metaphases. In this study, the effect of Mitomycin C, a recognized clastogen on human lymphocytes, was assayed in cultures of canine lymphocytes, an animal species that is flourishing in comparative medicine. Blood cultures were performed on samples from a male and a female dog. Cytotoxicity using the mitotic index and genotoxicity testing were performed with 0.25 µg/ml Mitomycin C. The total chromosomal aberrations were significantly higher due to the effect of Mitomycin C (P=0.0247). The number of chromatid breaks nearly quintupled, while pulverized metaphases were found to be six times more frequent, and endoduplicated cells were three times higher than in negative control cultures. The quantitatively most relevant chromosomal aberration was the presence of sticky metaphases, related to adverse effects in chromatin proteins. The potential use of canine lymphocytes for chromosomal aberration assay is emphasized in the evaluation or re-evaluation of the genotoxic in vitro effect of xenobiotics, to evidence chromosomal damage.
Keywords
canine cells; cytogenetics; clastogenic agents; chromosomal damage; lymphocyte cultures; mitomycin C
Hrčak ID:
307410
URI
Publication date:
16.8.2023.
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