Original scientific paper

https://doi.org/10.5513/JCEA01/24.3.3955

Allergen coding genes and miRNA-based DNA fingerprints in revealing differences among Vaccinium corymbosum L. and Vaccinium myrtillus L.

Jana ŽIAROVSKÁ ; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic *
Katarína RAŽNÁ ; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Lucia URBANOVÁ ; Slovak University of Agriculture in Nitra, Research Centre of AgroBioTech, Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Lucia KLONGOVÁ ; Slovak University of Agriculture in Nitra, Research Centre of AgroBioTech, Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Adam KOVÁČIK ; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Matúš KUČKA ; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Ľubomír HARENČÁR ; Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic
Lucia ZELEŇÁKOVÁ ; Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Institute of Food Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic

* Corresponding author.

Abstract

DNA-based marker fingerprinting techniques are developed with the aim of their effective use in the identification or comparative studies of plant genomes. In the genus Vaccinium, DNA markers help resolve taxonomic relationships among the species included in it. In this study, the allergen coding genes and micro RNAs were used as DNA markers and applied for the characterization of the fingerprints of Vaccinium corymbosum L. and Vaccinium myrtilus L. Both techniques were found to be effective in distinguishing the analysed Vaccinium accessions and in the generating of polymorphism and in the case of Bet v 1 Based Amplicon Polymorphism and Profilin Based Amplicon Polymorphism, separated fingerprints were obtained for Vaccinium myrtilus L.

Keywords

blueberries; allergen homologs DNA profiling; miRNA markers

Hrčak ID:

308204

URI

https://hrcak.srce.hr/308204

Publication date:

8.9.2023.

Article data in other languages: slovak

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