Original scientific paper

https://doi.org/10.5562/cca3984

Binding Parameters of Glucose to Human Serum Albumin in the Presence of Cinnamaldehyde and Cinnamic Acid from Cinnamon Bark (Cinnamomum verum)

Rony Abou-Khalil orcid.org/0000-0002-0214-242X ; Department of Chemistry and Biochemistry, Faculty of Arts and Sciences, Holy Spirit University of Kaslik - Jounieh, Lebanon *
Roger Matta ; Department of Chemistry and Biochemistry, Faculty of Arts and Sciences, Holy Spirit University of Kaslik - Jounieh, Lebanon
Elissar El-Hayek ; Department of Chemistry and Biochemistry, Faculty of Arts and Sciences, Holy Spirit University of Kaslik - Jounieh, Lebanon

* Corresponding author.

Abstract

Eugenol, cinnamic acid and cinnamaldehyde are the main active components of essential oils of cinnamon (Cinnamomum verum). This article studies their interaction with human serum albumin (HSA). Binding site number was calculated. Thermodynamic parameters (Ka, ΔrH°, ΔrG° and ΔrS°) were also determined. The binding of glucose to HSA in the presence and absence of cinnamaldehyde, cinnamic acid and eugenol was studied by fluorescence spectroscopy. Cinnamaldehyde possesses the higher binding constant (Ka) values followed by cinnamic acid. The thermodynamic parameters indicated hydrophobic interactions for cinnamaldehyde, van der Waals interaction and hydrogen bonding for cinnamic acid. The values of binding constant Ka and binding site number (n), of glucose to HSA determined by Scatchard method, increased only in the presence of cinnamaldehyde and/or cinnamic acid. Cinnamaldehyde and/or cinnamic acid modify the structure of HSA therefore increasing the binding of glucose to the protein.

Keywords

human serum albumin; glucose; cinnamaldehyde; cinnamic acid; eugenol

Hrčak ID:

311064

URI

https://hrcak.srce.hr/311064

Publication date:

10.12.2023.

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