Original scientific paper

Rapid Voltammetric Identification and Determination of Simvastatin at Trace Levels in Pharmaceuticals and Biological Fluid

Biljana Nigović ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Šebojka Komorsky-Lovrić ; Center for Marine and Environmental Research, Ruđer Bošković Institute, Zagreb, Croatia
Dubravka Devčić ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia

Abstract

Simvastatin is used as an antilipemic agent through inhibition of 3-hydroxy-3-methylglutarylcoenzyme
A reductase. The reduction behavior of drug has been studied in different supporting
electrolytes within the pH range 3–9. Based on the adsorptive character of simvastatin onto the
mercury electrode surface, a new cathodic square-wave stripping voltammetry method for its
direct determination at trace levels has been proposed. The voltammetric response could be
used to determine drug concentration in the range 1.0 × 10–8 to 7.5 × 10–7 mol L–1, with detection
limit of 4.5 × 10–9 mol L–1. Applicability to biological fluid analysis was illustrated. The
method was successfully employed, without sample pretreatment, for quantitation of the active
ingredient simvastatin in pharmaceuticals. The use of abrasive stripping voltammetry for rapid
identification of this drug in pharmaceutical dosage forms using a paraffin-impregnated graphite
electrode has been examined.

Keywords

simvastatin; square-wave voltammetry; abrasive stripping voltammetry; pharmaceuticals; biological fluid

Hrčak ID:

31133

URI

https://hrcak.srce.hr/31133

Publication date:

15.11.2008.

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