Short communication, Note

Randomly amplified polymorphic DNA-polymerase chain reaction fingerprinting of Babesia bigemina isolates of India - short communication

Reghu Ravindran orcid.org/0000-0001-9250-0552 ; Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U. P. India
Ashok Kumar Mishra ; Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U. P. India
Jammi Raghavendra Rao ; Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U. P. India

Abstract

This short communication describes the randomly amplified polymorphic DNA fingerprinting of B. bigemina from two geographically distant locations of the Indian subcontinent viz, Wayanad (Kerala state, Southern India) and Izatnagar (Uttar Pradesh State, Northern India). A total of four isolates, two each from Wayanad (Kerala, South India) and Izatnagar (North India) were used in the present study. RAPD-PCRs were carried out using four random oligonucleotide primers. Depending on the parasite isolate-primer combination, between one and five fragments in the range of 257 bp and 1948 bp were amplified. DNA fragments which can differentiate the two isolates were identified.

Keywords

randomly amplified polymorphic DNA (RAPD) PCR; Babesia bigemina; cattle; India

Hrčak ID:

31709

URI

https://hrcak.srce.hr/31709

Publication date:

18.12.2008.

Article data in other languages: croatian

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