Original scientific paper
https://doi.org/10.5599/jese.2036
Development of electrode by using gold-platinum alloy nanoparticles for electrochemical detection of serum amyloid A protein
Kalpana Ladi
; The NorthCap University, Applied Science Department, Gurugram, Haryana 122017, India
Aditya Sharma Ghrera
; The NorthCap University, Applied Science Department, Gurugram, Haryana 122017, India
Abstract
Gold-platinum alloy nanoparticles (AuPtNP) were electrochemically deposited on the surface of indium-tin-oxide (ITO) modified with (3-aminopropyl)triethoxysilane (APTES), after optimizing deposition conditions for the electroplating solution and number of deposition cycles. Different part ratios of Au/Pt used were 4/0, 3/1, 2/2, 1/3 and 0/4 (AuNP, Au3Pt1, Au2Pt2, Au1Pt3 and PtNP, respectively) in preparation of 1 mM solutions. FE-SEM, EDAX and XRD surface characterization techniques were used to confirm the presence of deposited AuPt alloy nanoparticles on the modified ITO surface. Electrochemical methods (CV, DPV and EIS) were used to investigate the electrochemical properties of prepared electrodes in the presence of ferri/ferrocyanide redox couple, which indicated the following increasing order of electrocatalytic peak current: Au < Au2Pt2 < Pt < Au1Pt3 < Au3Pt1. The most active electrode, Au3Pt1NP/APTES/ITO (with Au/Pt part ratio 3:1), was further used to fabricate the immunoelectrode SAA-Ab/Au3Pt1NP/APTES/ITO. The prepared immunoelectrode was tested for detection of serum amyloid A protein biomarker (APO-SAA) by immobilising SAA-specific antibodies (SAA ½ Ab) on its surface. Sensing studies on this immunoelectrode, performed by DPV technique, revealed the SAA biomarker detection in the linear range of 10 to 106 fg ml-1. The limit of detection was calculated as 7.0 fg ml-1.
Keywords
Electrochemical biosensing; bimetallic nanoparticles; electrocatalysts; immunoelectrode; inflammatory biomarker
Hrčak ID:
319749
URI
Publication date:
23.7.2024.
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