Original scientific paper

https://doi.org/10.24099/vet.arhiv.2479

Detection and characterization of canine corona virus infection: an enteric approach

Kanisht Batra ; Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
Praveen Kumar ; Veterinary Clinical Complex, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
Anju Sehrawat ; Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
Divya Agnihotri ; Veterinary Clinical Complex, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
Sushila Maan ; Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India *

* Corresponding author.

Abstract

Canine corona virus (CCoV) infection is a new emerging disease affecting dogs. It results in low mortality but considerable morbidity in dogs with intermediate to moderate enteritis. In India, serological research on dogs has revealed the existence of antibodies, demonstrating the prevalence of the condition among canines. The aim of the present study was to investigate the prevalence of CCoV infection in dogs suffering from gastroenteritis, and genetic analysis of circulating virus strains. The current study was designed to test fecal samples of dogs with gastroenteritis suspected for the presence of CCoV. Using primers based on the M-gene, PCR was used to amplify the RNA (c-DNA) templates retrieved from the fecal samples. RT-PCR based detection of CCoV showed positivity of 30% (15/50). The phylogentic analysis revealed that two samples had nt/aa identity levels of 100%/100% to the CCoV isolate from China and 99.19/100% of nt/aa identity with that from Korea. One of the samples showed divergence and revealed 95.63%/94.37% nt/aa identity with the China isolate and 95.15%/97.06% nt/aa identity with Indian isolates. The recombination analysis indicated a mutation rate in one of the samples. In brief, the present study illustrates the circulation of canine corona virus in dogs suffering from gastroenteritis. The present findings emphasize the need for vaccination of dogs for prevention, as a high prevalence of viral gastroenteritis was found in these dogs.

Keywords

RT-PCR; canine corona virus; diarrhea; phylogenetic analysis; genetic characterization

Hrčak ID:

322075

URI

https://hrcak.srce.hr/322075

Publication date:

1.11.2024.

Article data in other languages: croatian

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