Original scientific paper
https://doi.org/https://doi.org/10.5599/jese.2329
Fabrication of a molecularly imprinted poly(methyl methacryl-ate) decorated graphite electrode for detection of aloe-emodin in Aloe vera
Milan Dhara
; Department of ECE, Future Institute of Engineering and Management, Kolkata, India
Sanjoy Banerjee
; Department of ECE, Future Institute of Engineering and Management, Kolkata, India
Barnali Ghatak
; Department of ECE, Aliah University, Kolkata, India
Hemanta Naskar
; IEM, University of Engineering and Management, Kolkata, India
Amit Kumar Chakraborty
; Department of Physics, NIT, Durgapur, India
Nityananda Das
; Department of Physics, JKC, Sidho-Kanho-Birsha University, Purulia, India
Chezhiyan Kuppusamy
; National Food Laboratory, Kolkata, India
Gyan Prakash Sharma
; National Food Laboratory, Kolkata, India
Rajib Bandyopadhyay
; Department of IEE, Jadavpur University, Kolkata, India
Bipan Tudu
orcid.org/0000-0001-9323-4276
; Department of IEE, Jadavpur University, Kolkata, India
Abstract
In this work, the authors represent a comprehensive approach for the development and validation of a novel graphite-supported molecularly imprinted poly(methyl methacrylate) electrode for the selective and sensitive detection of aloe-emodin (AE) in aloe-based cosmetic products. The electrocatalytic activity of the electrode was evaluated using cyclic voltammetry and differential pulse voltammetry techniques in phosphate buffer saline. Surface morphology, structural characteristics, and imprinting endorsement were investigated using field emission scanning electron microscopy, Fourier-transform infrared spectroscopy, and UV-Vis spectroscopy. Under the optimal conditions, the electrode showed an oxidative differential pulse voltammetry peak at -0.2931±0.011 V (vs. Ag/AgCl, saturated KCl). The current was increased linearly with increasing concentrations of AE from 0.0005 to 350 µM, with a low detection limit of 0.0003 µM. The voltammetric analysis has been validated by reverse-phase high-performance liquid chromatography (RP-HPLC) methods. A partial least square regression model was developed to correlate the results obtained from the proposed system with the reference values obtained by RP-HPLC. The model showed a high prediction accuracy of 95.95 % for the detection of AE in cosmetic samples. The developed electrode provides a low-cost, rapid, easy, and convenient method for the detection of AE in cosmetic products, offering potential benefits for quality control and safety assessment in the cosmetic industry.
Keywords
Hrčak ID:
323674
URI
Publication date:
26.10.2024.
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