Original scientific paper
Serum Antibodies Positivity to 12 Helicobacter pylori Virulence Antigens in Patients with Benign or Malignant Gastroduodenal Diseases – Cross-sectional Study
Tajana Filipec Kanižaj
; Department of Internal Medicine, University Hospital Merkur, Zagreb, Croatia
Miroslava Katičić
; Department of Internal Medicine, University Hospital Merkur, Zagreb, Croatia
Vladimir Presečki
; Depatrment of Microbiology and Parasitology, University of Zagreb, School of Medicine, Zagreb, Croatia
Abstract
Aim To investigate the association of gastric histological and
endoscopic findings in patients with Helicobacter pylori (H. pylori),
according to presence of seropositivity to 12 bacterial virulence
antigens.
Methods This is a cross-sectional single-center study of 360
consecutive outpatients referred in the period of one year to
upper gastrointestinal endoscopy because of dyspeptic complaints.
Patients sera were tested by Western blot method to
determine the presence of serum antibodies to bacterial virulence
antigens – p120 (CagA – cytotoxin-associated antigen),
p95 (VacA – vacuolating cytotoxin), p67 (FSH – flagellar sheath
protein), p66 (UreB – urease enzyme heavy subunit), p57 (HSP
homologue – heath shock protein homologue), p54 (flagellin),
p33, p30 (OMP – outer membrane protein), p29 (UreA – urease
enzyme light subunit), p26, p19, and p17. Upper gastrointestinal
endoscopy was performed, endoscopic diagnosis recorded,
and 4 mucosal biopsy samples were obtained and assessed according
to Updated Sydney protocol.
Results The sera of 207 patients were analyzed. Thirty patients
had gastric adenocarcinoma, 126 peptic ulcers, and 51 normal
finding. p120 (CagA) seropositivity was significantly more
often present in patients with higher activity grade in the antrum
(P = 0.025), p30 in patients with greater inflammation in
the antrum (P = 0.025) and the corpus (P = 0.010), p33 in patients
with greater inflammation in the corpus (P = 0.050), and
p19 (OMP) in patients with lower intestinal metaplasia grades
in the corpus (P = 0.025). Seroreactivity to all other bacterial proteins
showed no association with the histological status of the
stomach mucosa. Except for the seropositivity to protein p95
(VacA), which was more often present in patients with duodenal
ulcer (P = 0.006), there was no difference in seroreactivity to
other bacterial proteins and upper gastrointestinal endoscopic
findings.
Conclusions p120 (CagA), p33, p 30 (OMP), and p19 (OMP)
seropositivity was more often present in patients with higher
grades of the histological parameters of gastritis and seropositivity
to protein p95 (VacA) with endoscopic presence of duodenal
ulcer. Histological parameters of gastritis are more associated
with bacterial virulence than endoscopic findings.
Keywords
Hrčak ID:
38715
URI
Publication date:
15.4.2009.
Visits: 1.230 *