Original scientific paper

https://doi.org/10.2478/v10007-009-0035-6

Development of a radiolabeled β-human chorionic gonadotropin

AMIR R. JALILIAN ; Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS), Karaj, Iran
MOHAMMAD REZA KHOSHDEL ; Islamic Azad University, Science and Research Branch, P. O. Box 14155-775, Tehran, Iran
JAVAD GAROUSI ; Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS), Karaj, Iran
HASSAN YOUSEFNIA ; Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS), Karaj, Iran
MOHAMMAD A. HOSSEINI ; Islamic Azad University, Science and Research Branch, P. O. Box 14155-775, Tehran, Iran
SAEED RAJABIFAR ; Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS), Karaj, Iran
DARYOUSH SARDARI ; Islamic Azad University, Science and Research Branch, P. O. Box 14155-775, Tehran, Iran

Abstract

-human chorionic gonadotropin (-hCG) was successively labeled with [67Ga] gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 2223 TBq mM1, labeling efficiency 80 %). Preliminary in vivo studies (ID g1, %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported -hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively.

Keywords

beta-hCG; radiolabeling; biodistribution; radiopharmaceutical

Hrčak ID:

40497

URI

https://hrcak.srce.hr/40497

Publication date:

1.12.2009.

Article data in other languages: croatian

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