Review article

Sample preparation and RPHPLC determination of diuretics in human body fluids

DRAGICA ZENDELOVSKA
TRAJČE STAFILOV

Abstract

This article described reverse phase high-performance liquid chromatography (RPHPLC) methods for determination of diuretics in different human body fluids (whole blood, plasma, serum or urine). Sample preparation procedures including solid-phase extraction, liquid-liquid extraction, dilution, precipitation as well as automated RPHPLC procedures were discussed in order to give advantages and disadvantages of each type of sample preparation. Also, values for analytical recovery of each procedure used for sample preparation are summarized. The most important RPHPLC parameters (detection mode, stationary phase, mobile phase, sensitivity, etc.) are also summarized and discussed.

Keywords

diuretics; RPHPLC; determination; human body fluids

Hrčak ID:

4548

URI

https://hrcak.srce.hr/4548

Publication date:

1.6.2006.

Article data in other languages: croatian

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