Original scientific paper

Genetic characterization of porcine circovirus type 2 isolated from different pig-farms in Croatia

Nevenka Rudan ; Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Charlotte Kristiane Hjulsager ; National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark
Kitt Dupont ; National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark
Branko Bačanek ; Croatian Veterinary Institute Zagreb, Regional Veterinary Laboratory, Križevci, Croatia
Evica Marković ; Ministry of Agriculture, Fisheries and Rural Development, Zagreb, Croatia
Josip Madić ; Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

Histopathological findings in 25 pig tissue samples, which indicated PCVD (porcine circovirus diseases), were studied. Pig tissue samples originated from 5 different pig-farms in the north-west part of Croatia. Histopathological lesions showed two clinical pictures of the disease: porcine multisystemic wasting syndrome (PMWS) and porcine dermatitis nephropathy syndrome (PDNS). All samples were tested by PCR for the presence of porcine circovirus type 2 (PCV2). Twenty of them were PCV2 positive. PCV2 DNA was quantified
by realtime-PCR in all twenty samples with a wide range of PCV2 loads ranging from 105 to 1010 PCV2 genomes/μL. The highest PCV2 loads were detected in pigs with PMWS lesions, while PDNS affected pigs had lower PCV2 loads. The 20 PCV2 isolates were sequenced and analyzed. Nineteen isolates belonged to PCV2 group 1 and only one to PCV2 group 2. We found no link between genotypes and clinical form of the disease.

Keywords

porcine circovirus 2; PCV2; real-time PCR; genotyping

Hrčak ID:

45595

URI

https://hrcak.srce.hr/45595

Publication date:

10.7.2009.

Article data in other languages: croatian

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