Acta Pharmaceutica, Vol. 56 No. 4, 2006.
Short communication, Note
Optimized determination of lorazepam in human serum with extraction and high-performance liquid chromatographic analysis
AMIR G. KAZEMIFARD
KHEIROLLAH. GHOLAMI
ALIREZA DABIRSIAGHI
Abstract
The present research was designated to evaluate a rapid and sensitive method for determining low concentrations of the highly active drug lorazepam in serum. Isolation of the drug from biological fluid after addition of nordazepam as the internal standard was achieved using a simple liquid-liquid extraction with dichloromethane and the extracted compounds were quantified by high-performance liquid chromatography. Chromatographic separation on a reversed phase column containing a stationary phase with low silanol activity resulted in a perfectly symmetrical peak with a tailing factor of 1.0. The limit of quantitation in serum is 2.5 ng mL-1 for both lorazepam and internal standard. The procedure is rapid and sensitive enough for determination of lorazepam in serum.
Keywords
lorazepam; serum; high-performance liquid chromatography; peak tailing
Hrčak ID:
4610
URI
Publication date:
1.12.2006.
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