Original scientific paper

Spectrofluorimetric determination of ciclopirox olamine via ternary complex with Tb(III) and EDTA

MOHAMED IBRAHIM WALASH
MOHAMED SALEM RIZK
MANAL IBRAHIM EID
MONA EL SAYED FATHY

Abstract

A highly sensitive and selective spectrofluorimetric method was developed for the determination of ciclopirox olamine in raw material and in dosage forms. The proposed method is based on the formation of a ternary complex with Tb(III) in the presence of ethylenediaminetetraacetic acid. It was found that this complex manifests intense fluorescence at λem 489 and 545 nm with excitation at 295 nm. Different experimental parameters affecting the fluorescence intensity of the complex were carefully studied and incorporated into the procedure. Under the described conditions, the method is applicable over the concentration range of 30150 and 1070 ng mL-1 with minimum detectability of 6.7 and 0.9 ng mL-1 at λem 489 and 545 nm, respectively. The mean percentage recovery at λem 489 and λem 545 nm ranged between 98.7 and 100.2 for the pure substance, solution, and cream. Relative error of 0.10.4% and RSD up to 0.9% were estimated at λem 489 and 545 nm. A proposal of the reaction pathway is given.

Keywords

ciclopirox olamine; Tb(III); EDTA; spectrofluorimetry; dosage forms

Hrčak ID:

4612

URI

https://hrcak.srce.hr/4612

Publication date:

1.12.2006.

Article data in other languages: croatian

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