Original scientific paper

https://doi.org/10.2478/v10007-010-0007-x

Isolation and of free-radical-scavenging properties of cyanidin 3-O-glycosides from the fruits of Ribes biebersteinii Berl.

ABBAS DELAZAR ; School of Pharmacy, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
LALEH KHODAIE ; School of Pharmacy, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
JALIL AFSHAR ; School of Pharmacy, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
LUTFUN NAHAR ; Drug Discovery and Design Research Division, Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, MA Building, Wulfruna Street, Wolverhampton WV1 1LY, West Midland, UK
SATYAJIT D. SARKER ; Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, MM Building, Molineux Street, Wolverhampton WV1 1SB, West Midland, UK

Abstract

The reversed-phase preparative high performance liquid chromatographic purification of the methanol extract of the fruits of Ribes biebersteinii Berl. (Grossulariaceae) afforded five cyanidin glycosides, 3-O-sambubiosyl-5-O-glucosyl cyanidin (1), cyanidin 3-O-sambubioside (2), cyanidin 3-O-glucoside (3), cyanidin 3-O-(2G-xylosyl)-rutinoside (4) and cyanidin 3-O-rutinoside (5). They showed considerable free-radical-scavenging properties in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay with the RC50 values of 9.29 × 106, 9.33 × 106, 8.31 × 106, 8.96 × 106 and 9.55 × 106 mol L1, respectively. The structures of these compounds were elucidated by various chemical hydrolyses and spectroscopic means. The total anthocyanin content was 1.9 g per 100 g dried fruits on cyanidin 3-glucoside basis.

Keywords

Ribes biebersteinii Berl. (Grossulariaceae); 2,2-diphenyl-1-picrylhydrazyl (DPPH); cyanidin glycoside; free-radical-scavenger

Hrčak ID:

48281

URI

https://hrcak.srce.hr/48281

Publication date:

1.3.2010.

Article data in other languages: croatian

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