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Croatian Medical Journal, Vol. 50 No. 6, 2009.

Original scientific paper

ABO Blood Groups and Genetic Risk Factors for Thrombosis in Croatian Population

Irena Jukić orcid id orcid.org/0000-0003-2778-8400 ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Jasna Bingulac-Popović orcid id orcid.org/0000-0002-9449-3154 ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Vesna Đogić orcid id orcid.org/0000-0002-6025-4649 ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Ivana Babić orcid id orcid.org/0000-0003-0976-769X ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Jelena Culej ; Department of Clinical Chemistry, Dr Fran Mihaljevic University Hospital for Infectious Diseases, Zagreb, Croatia
Maja Tomičić ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Tomislav Vuk orcid id orcid.org/0000-0001-9951-6119 ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Dorotea Šarlija ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
Melita Balija ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia

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Abstract

Aim To assess the association between ABO blood group
genotypes and genetic risk factors for thrombosis (FV
Leiden, prothrombin G20210A, and methylenetetrahydrofolate
reductase C677T mutations) in the Croatian population
and to determine whether genetic predisposition to
thrombotic risk is higher in non-OO blood group genotypes
than in OO blood group genotypes.
Methods The study included 154 patients with thrombosis
and 200 asymptomatic blood donors as a control group.
Genotyping to 5 common alleles of ABO blood groups was
performed by polymerase chain reaction with sequence
specific primers (PCR-SSP). FV Leiden was determined by
PCR-SSP, while prothrombin and methylenetetrahydrofolate
reductase were determined by PCR and restriction
fragment length polymorphism (PCR-RFLP).
Results There was an association between non-OO blood
group genotypes and the risk of thrombosis (odds ratio
[OR] 2.08, 95% confidence interval [CI], 1.32-3.27). The
strongest association with thrombotic risk was recorded
for A1B/A2B blood group genotypes (OR, 2.73; 95% CI, 1.10-
6.74), followed by BB/O1B/O2B (OR, 2.29; 95% CI, 1.25-4.21)
and O1A1/O2A1 (OR, 1.95; 95% CI, 1.15-3.31). FV Leiden increased
the risk of thrombosis 31-fold in the group of OO
carriers and fourfold in the group of non-OO carriers. There
was no significant difference in the risk of thrombosis between
OO and non-OO blood groups associated with prothrombin
mutation. Non-OO carriers positive for methylenetetrahydrofolate
reductase had a 5.7 times greater risk
of thrombosis than that recorded in OO carriers negative
for methylenetetrahydrofolate reductase.
Conclusion Study results confirmed the association of
non-OO blood group genotypes with an increased risk of
thrombosis in Croatia.

Keywords

ABO blood groups; Risk of thrombosis; Croatian population; Genetic risk factors for thrombosis; ABO blood genotypes; FV Leiden; prothrombin G20210A; MTHFR

Hrčak ID:

48553

URI

https://hrcak.srce.hr/48553

Publication date:

15.12.2009.

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