Veterinary Archives, Vol. 76 No. 1, 2006.
Original scientific paper
The efficacy of gradient Percoll® on bull sperm separation for in vitro fertilization
Marko Samardžija
Tomislav Dobranić
Martina Karadjole
Iva Getz
Silvijo Vince
Damjan Gračner
Nino Mačešić
Ivana Filaković
Abstract
In the present study we examined the effect of density gradient preparation Percoll® on bull sperm separation and in vitro fertilization (IVF) results. Frozen/thawed semen from 5 Simmental bulls was pooled and sperm quality parameters were evaluated before and after sperm separation using Percoll® density gradient preparation. We evaluated sperm motility (subjective), sperm concentration (Thoma chamber) and sperm membrane activity by HOS assay. Initial sperm parameters results were as follows: motility 50%, sperm concentration 80.80 x 106 spz/mL and functional integrity of sperm cells 39.25%. Final sperm motility was 63%, final sperm concentration 28 x 106 spz/mL and HOS assay results after Percoll® gradient was 57.60%. A total of 211 oocytes were matured and fertilized in vitro and cultured in SOFaaBSA in five repetitions. The cleavage (D2), blastocysts (D7) and hatched blastocysts (D10) were 63.13%, 20.38%, and 10.64%, respectively. We found no significant correlations between sperm parameters and IVF results (r<0.5; P>0.05). It is possible to conclude that sperm evaluation parameters after sperm separation by gradient Percoll® are not reliable in predicting the outcome of IVF. However, using gradient Percoll® for sperm separation in IVF is necessary to improve the quality of separated sperm able to significantly improve sperm quality with a high rate of progressive motility and morphological normal spermatozoa.
Keywords
bull; sperm; fertilization; Percoll®
Hrčak ID:
5083
URI
Publication date:
20.2.2006.
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