Veterinary Archives, Vol. 76 No. 1, 2006.
Original scientific paper
Isoenzymatic pattern of glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase in Iranian Echinococcus granulosus
Seyyed Hossein Hosseini
Malihe Pourkabir
Farzad Asadi
Karen Lee
Hossein Razijalali
Abstract
Morphology and genetic studies of Echinococcus granulosus have indicated that there are 2 different strains of this parasite in Iran, ovine and camel. However, no study has been carried out to date on the phenotypic characterization of this parasite. In the present study the electrophoretic pattern of glucose-6-phosphate dehydrogenase (G6PD) and isocitrate dehydrogenase (ICD) was demonstrated. In this study isolates of ovine and camel hydatid cysts were collected from slaughterhouses across Iran. Hydatid fluid and hydatid sand were separated and collected. The fluid was concentrated and the protoscoleces from the sand were extracted. The amount of total protein in protoscoleces and concentrated hydatid fluid was determined. Electrophoretic pattern of extract was indicated by SDS-PAGE. Non-denaturating electrophoresis was also used for study of electrophoretic pattern of G6PD and ICD; so that major and minor enzyme activities were indicated. Densitometry of electrophoretic pattern indicated 2 major bands for each of these enzymes in camel and sheep with the same pattern in the extract of protoscoleces and hydatid fluid. Based on the fact that the band of enzymes in each of 2 isolates has different molecular patterns; we propose that these represent the 2 different strains (sheep-dog and camel-dog) of this parasite in Iran.
Keywords
Echinococcus granulosus; enzyme; phenotype; camel; sheep
Hrčak ID:
5084
URI
Publication date:
20.2.2006.
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