Original scientific paper
Expression of Bone Morphogenetic Protein-7, It´s Receptors and Smad1/5/8 in Normal Human Kidney and Renal Cell Cancer
Dean Markić
; Department of Urology, University Hospital of Rijeka, Rijeka, Croatia
Tanja Ćelić
; Department of Anatomy, School of Medicine, University of Rijeka, Rijeka, Croatia
Josip Španjol
; Department of Urology, University Hospital of Rijeka, Rijeka, Croatia
Antun Gršković
; Department of Urology, University Hospital of Rijeka, Rijeka, Croatia
Dragica Bobinac
; Department of Anatomy, School of Medicine, University of Rijeka, Rijeka, Croatia
Željko Fučkar
; Department of Urology, University Hospital of Rijeka, Rijeka, Croatia
Abstract
Bone morphogenetic proteins (BMPs) are cytokines which are important for kidney homeostasis but also have role in the some renal diseases and renal cell carcinoma (RCC)1–5. In the last three decades incidence of RCC was constantly increased and the role of different molecular biomarkers in RCC is explored1. We analyzed expression of BMP-7, their receptors (BMPR-IA, BMPR-IB, BMPR-II) and proteins of their signaling pathway (pSmad1/5/8) in sixteen renal cancer samples and paired normal tissue. Tissue samples were analyzed by immunohistochemistry and Western blot. BMP-7, BMP receptors and pSmad1/5/8 were expressed in all structures of normal kidney but dominantly in the proximal tubular cells. In the cancer samples their expression was also noticed. Comparison of BMPs between different tissue showed increased expression of BMPR-IB and pSmad 1/5/8 and decreased expression of BMP-7 and BMPR-II in RCC compared to normal kidney. BMPR-IA was detected with immunohistochemistry but with Western blot attenuated signal was presented. BMP-7, BMP receptors and pSmad1/5/8 were showed in normal kidney and RCC. Detected alterations of BMP-7, BMP receptors and pSmad expression in RCC suggested their possible role in tumorigenesis of kidney cancer.
Keywords
kidney; bone morphogenetic proteins; renal cell carcinoma; epidemiology; pathology
Hrčak ID:
51648
URI
Publication date:
20.4.2010.
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