Original scientific paper
THE EFFECT OF CLPS AND RYR1 GENE POLYMORPHISM ON MEAT QUALITY OF ZŁOTNICKA SPOTTED PIGS
Wojciech KAPELAŃSKI
; Department of Pig Breeding, University of Technology and Life Sciences in Bydgoszcz, Poland
Anna WILKANOWSKA
; Department of Pig Breeding, University of Technology and Life Sciences in Bydgoszcz, Poland
Aleksandra CEBULSKA
; Department of Pig Breeding, University of Technology and Life Sciences in Bydgoszcz, Poland
Milena BIEGNIEWSKA
; Department of Pig Breeding, University of Technology and Life Sciences in Bydgoszcz, Poland
Abstract
The aim of the study was to relate the polymorphisms at CLPS and RYR1 genes to pork quality in case of Złotnicka Spotted breed. The experiment was carried out on 30 purebred Złotnicka Spotted pigs, which were reared in standard conditions and slaughtered at approximately 100 to 110 kg body weight. Genetic polymorphisms were determined with the PCR-RFLP procedure. Polymorphism at the colipase gene (CLPS) was identified with DdeI restriction enzyme
and stress sensibility gene (RYR1) with HinPI. In the studied pig sample three genotypes at locus CLPS/DdeI and two genotypes at locus RYR1/HinPI were found. The genotype frequencies at CLPS gene were 0.07 for AA, 0.40 for AB and 0.53 for BB. Colipase gene affected intramuscular fat content in RYR1/CT pigs. Meat from pigs with genotype BB had higher intramuscular fat content (2.50%) compared to AA group (1.59%); P≤0.05. Moreover, significant influence of gene RYR1T on pH1 trait was shown. Values of all analyzed traits were within ranges characterizing good meat
quality of the native Polish Złotnicka Spotted pig.
Keywords
pigs; meat quality; CLPS gene; RYR1 gene
Hrčak ID:
55706
URI
Publication date:
30.6.2010.
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