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Original scientific paper

Endocrine profifi le and testicular histomorphometry in neonatal rats of diabetic mothers

Gholamali Jelodar ; Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Zabiholah Khaksar ; Department of Histology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mohammad Pourahmadi ; Department of Anatomy and Histology Jahrom, University of Medical Sciences, Iran


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Abstract

This study was conducted to evaluate the effects of maternal diabetes on foetal testicular structure and function, and reproductive hormone level. Sixteen adult female rats were divided into two groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. Blood was collected from 30-day old male offspring and the level of testosterone, FSH and LH measured in their serum. The weight and volume of the testes and various histological parameters of the testes were determined. The results showed a significant decrease in LH, FSH and testosterone in the sera of offspring from diabetic mothers (ODM) compared with the control group.Weight and volume of testes were approximately 30% and 27.5% respectively less than those of the control group (P<0.05). In histological sections the number of seminiferous tubules (ST) increased significantly (28.9%) (P<0.05), whilst thickness of the testicular capsule (-17%), the number of Leydig cells (-28%), Sertoli cells (-11%), spermatogonia (-11%) and the diameter of ST (-21.8%) showed signififi cantly reduced values in the ODM compared to controls (P<0.05). In conclusion maternal hyperglycemia has a deleterious effect on testicular parameters during fetal life, which will affect reproductive endocrinology in the postnatal period and may impact on fertility.

Keywords

maternal diabetes; testis; rat; histomorphometry; endocrine

Hrčak ID:

58457

URI

https://hrcak.srce.hr/58457

Publication date:

16.6.2010.

Article data in other languages: croatian

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