Professional paper

Review of Detection of Brucella spp. by Polymerase Chain Reaction

Wei Ling Yu ; Ottawa Laboratories (Fallowfield), Canadian Food Inspection Agency, Nepean, Ontario, Canada
Klaus Nielsen ; Ottawa Laboratories (Fallowfield), Canadian Food Inspection Agency, Nepean, Ontario, Canada

Abstract

Here we present a review of most of the currently used
polymerase chain reaction (PCR)-based methods for identification
of Brucella bacteria in biological samples. We focused
in particular on methods using single-pair primers,
multiplex primers, real-time PCRs, PCRs for marine Brucella,
and PCRs for molecular biotyping. These methods are becoming
very important tools for the identification of Brucella,
at the species level and recently also at the biovar
level. These techniques require minimum biological containment
and can provide results in a very short time. In
addition, genetic fingerprinting of isolates aid in epidemiological
studies of the disease and its control. PCR-based
methods are more useful and practical than conventional
methods used to identify Brucella spp., and new methods
for Brucella spp. identification and typing are still being developed.
However, the sensitivity, specificity, and issues of
quality control and quality assurance using these methods
must be fully validated on clinical samples before PCR can
be used in routine laboratory testing for brucellosis.

Keywords

Brucellosis; diagnosis; polymerase chain reaction; single primers; multiplex; real-time; species; biotypes

Hrčak ID:

58578

URI

https://hrcak.srce.hr/58578

Publication date:

15.8.2010.

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