Veterinary Archives, Vol. 80 No. 5, 2010.
Original scientific paper
Development and evaluation of flow through technique for diagnosis of cystic echinococcosis in cattle
Narayanaperumal Jeyathilakan
orcid.org/0000-0002-2367-9808
; Department of Veterinary Parasitology, Madras Veterinary College, Chennai, India
Shahulhameed A. Basith
; Department of Veterinary Parasitology, Madras Veterinary College, Chennai, India
Lalitha John
; Department of Veterinary Parasitology, Madras Veterinary College, Chennai, India
Navamani D. J. Chandran
; TANUVAS, Chennai, India
Gopal D. Raj
; Department of Animal Biotechnology, Madras Veterinary College, Chennai, India
Abstract
A novel in vitro flow through technique was developed and evaluated for immunodiagnosis of cystic echinococcosis in cattle using hydatid specific non-cross reactive 8 kDa protein. The 8 kDa protein was prepared from hydatid cyst fluid by DEAE-Sepharose fast flow anion exchange chromatography. In this flow through technique, the 8 kDa antigen was coated on the nitrocellulose membrane of the flow through device. Protein A Colloidal gold was used as detector. The evaluation of the technique was performed by comparing 150 known positive hydatid serum and known negative serum collected from cattle. The test was shown to have high sensitivity and specificity that were closely correlated with those of Enzyme-linked immunoelctrotransfer blot. Furthermore, the immunofiltration based assay is rapid and easy to perform with no requirement of special skill, reagent or instrumentation. This suggests the flow through technique is an acceptable alternative for use in clinical laboratories lacking specialized equipment as well as large scale screening of cystic echinococcosis in
the field with both animal and human populations.
Keywords
cystic echinococcosis; flow through technique; 8 kDa antigen
Hrčak ID:
62142
URI
Publication date:
26.10.2010.
Visits: 1.306 *