Original scientific paper

Spectrophotometric Determination of Isoxsuprine Hydrochloride as Base Form in Pharmaceutical Formulation through Charge Transfer Complexation

Kanakapura Basavaiah ; Department of Chemistry, University of Mysore, Manasagangothri, Mysore-570006, India
Kalsang Tharpa ; Department of Chemistry, University of Mysore, Manasagangothri, Mysore-570006, India
Kanakapura Basavaiah Vinay ; Department of Chemistry, University of Mysore, Manasagangothri, Mysore-570006, India

Abstract

Two simple and selective spectrophotometric methods are described for the determination of
isoxsuprine hydrochloride (ISX·HCl) as base form in bulk drug and in injections and tablets. The methods
are based on the molecular charge-transfer complexation of ISX base (ISX) with either p-chloranilic acid
(PCA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). The intense colored radical anions formed
on dissociation, are quantitated at 520 nm (PCA method) or 600 nm (DDQ method). The optimum assay
conditions were optimized. Beer's law is obeyed in the concentration ranges 5.28 × 10–5 to 42.2 × 10–5 mol
dm–3 in PCA method and 0.79 × 10–5 to 10.6 × 10–5 mol dm–3 in DDQ method, with respective molar absorptivity
values of 1.32 × 103 and 6.55 × 103 dm3 mol–1 cm–1. The reaction stoichiometry in both methods
was evaluated by either Job’s method or limiting logarithmic method and was found to be 1:1 (ISX: PCA,
ISX: DDQ). Developed methods were validated according to ICH guidelines and found to be accurate and
precise.

Keywords

isoxsuprine hydrochloride; p-chloranilic acid; 2; 3-dichloro-5; 6-dicyano-1; 4-benzoquinone; spectrophotometry; injection; tablets

Hrčak ID:

62646

URI

https://hrcak.srce.hr/62646

Publication date:

1.12.2010.

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