Original scientific paper

Evaluation of Saponin and Montanide ISA 50 adjuvants for their immunopotency and effect on humoral immune response of calves to purified midgut antigen of Boophilus microplus.

Muthusamy Raman ; Department of Parasitology Veterinary College, and Research Institute, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu State, India
Pachaikani Ramadass ; Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, India
Govindasamy Rajavelu ; Centre for Animal Health Studies, TANUVAS, Chennai, India

Abstract

An immunization experimental study was conducted on eighteen cross-bred calves (Bos taurus x Bos indicus) using a gel chromatography purified midgut antigen of Boophilus microplus with two adjuvants, namely Saponin and Montanide ISA 50. Of the two adjuvants, a pronounced humoral immune response was observed by ELISA (1.428±0.061) with a significantly (P<0.01) higher rejection of ticks (48.03%) and low egg rate conversion efficacy (21.13) in the Saponin group of calves than in the Montanide group. Performance of the two adjuvants was assessed using various feeding and reproductive parameters of ticks collected from immunized and control animals. Nevertheless, the level of protection offered by Montanide ISA 50 was almost equal to the Saponin group, but with development of a mild local inflammatory reaction in immunized calves.

Keywords

Boophilus microplus; midgut antigen; Saponin; Montanide ISA 50; immunopotentiation effect; calves

Hrčak ID:

67846

URI

https://hrcak.srce.hr/67846

Publication date:

22.4.2004.

Article data in other languages: croatian

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