Original scientific paper
https://doi.org/10.2478/10004-1254-62-2011-2105
Genotoxic Effects of Green Tea Extract on Human Laryngeal Carcinoma Cells In Vitro
Ksenija Durgo
; Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Sandra Kostić
; Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Katarina Gradiški
; Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Draženka Komes
; Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Maja Osmak
; Ruđer Bošković Institute, Zagreb, Croatia
Jasna Franekić
; Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Abstract
Green tea (Camellia sinensis) contains several bioactive compounds which protect the cell and prevent tumour development. Phytochemicals in green tea extract (mostly flavonoids) scavenge free radicals, but also induce pro-oxidative reactions in the cell. In this study, we evaluated the potential cytotoxic and prooxidative effects of green tea extract and its two main flavonoid constituents epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) on human laryngeal carcinoma cell line (HEp2) and its crossresistant cell line CK2. The aim was to see if the extract and its two flavonoids could increase the sensitivity of the cisplatin-resistant cell line CK2 in comparison to the parental cell line. The results show that EGCG and green tea extract increased the DNA damage in the CK2 cell line during short exposure. The cytotoxicity of EGCG and ECG increased with the time of incubation. Green tea extract induced lipid peroxidation in the CK2 cell line. The pro-oxidant effect of green tea was determined at concentrations higher than those found in traditionally prepared green tea infusions.
Keywords
cell line; cytotoxicity; DNA damage; epicatechin gallate; epigallocatechin gallate; green tea; glutathione; lipid peroxidation
Hrčak ID:
69296
URI
Publication date:
17.6.2011.
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