Review article

New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks

Cai-xia Li ; Institute of Forensic Science, Ministry of Public Security, Beijing, China
Gui-qiang Wang ; Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing, China
Wan-shui Li ; Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing, China
Jiang-ping Huang ; Chinese Peoples Public Security University, Beijing, China
Annquan Ji ; Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing, China
Lan Hu ; Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing, China

Abstract

Aim To isolate mucosal cells of the perpetrator in a sexual
assault case from a complex mixture of his mucosal cells
and the victim’s skin by micromanipulation prior to genomic
analysis.
Methods To capture and analyze mucosal cells we used
the micromanipulation with on-chip low volume polymerase
chain reaction (LV-PCR). Consensus DNA profiles
were generated from 5 replicate experiments.
Results and conclusions We validated the use of micromanipulation
with on-chip LV-PCR for genomic analysis of
complex biological mixtures in a fatal rape case. The perpetrator’s
mucosal cells were captured from nipple swabs of
the victim, and a single-source DNA profile was generated
from cell mixtures. These data suggest that micromanipulation
with on-chip LV-PCR is an effective forensic tool for
the analysis of specific cells from complex samples.

Keywords

Hrčak ID:

71439

URI

https://hrcak.srce.hr/71439

Publication date:

15.6.2011.

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