Original scientific paper

https://doi.org/10.5562/cca1763

Separation of 1,4-benzodiazepine Derivates by Micellar Electrokinetic Capillary Chromatography Using Cyclodextrines as Buffer Modifiers

Gabriel Hancu ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Târgu Mureş, Gh. Marinescu 38, 540000 Târgu Mureş, Romania
Árpád Gyéresi ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Târgu Mureş, Gh. Marinescu 38, 540000 Târgu Mureş, Romania

Abstract

The applicability of micellar electrokinetic capillary chromatography (MEKC) for the separation
of benzodiazepine (BZD) derivates with different structural characteristics has been studied. Efforts were
first focused on the optimization of the analytical conditions, on the effects of buffer concentration, surfactant
concentration and buffer pH on the separation. To manipulate selectivity of the separation β-
cyclodextrin (CD) was added to the buffer solution, and urea was used to improve the solubility of the CD
in water. The CD-modified MEKC separation of the eight BZDs was achieved within 15 minutes using 25
mmol dm−3 natrium tetraborate-50 mmol dm−3 sodium dodecyl sulphate-15 mmol dm−3 β-CD-2 mol dm−3
urea (pH 9.3) as the running buffer. The proposed separation method was evaluated on the basis of precision,
linearity and limit of detection. (doi: 10.5562/cca1763)

Keywords

benzodiazepines; separation; capillary electrophoresis; micelles; cyclodextrines

Hrčak ID:

74771

URI

https://hrcak.srce.hr/74771

Publication date:

13.11.2011.

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