Original scientific paper

https://doi.org/10.5562/cca1777

Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

Anna Latoszek ; Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland
Carmen García-Ruiz ; Department of Analytical Chemistry and Chemical Engineering, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain
Maria Luisa Marina ; Department of Analytical Chemistry and Chemical Engineering, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain
Francisco J. de la Mata ; Department of Inorganic Chemistry, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain
Rafael Gómez ; Department of Analytical Chemistry and Chemical Engineering, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain
Beatriz Rasines ; Department of Analytical Chemistry and Chemical Engineering, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain
Alejandro Cifuentes ; CIBER-BBN and Department of Food Analysis, Institute of Fermentation Industry (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain
Ewa Poboży ; Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland
Marek Trojanowicz ; Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland

Abstract

The capillary electrophoresis with UV detection was employed for protein profiling in extracts
from maize and soybeans. Modifications of back-ground electrolyte and coating the capillary wall with
polybrene was employed in order to decrease the protein adsorption on the capillary walls. The obtained
protein profiles were compared for transgenic and non-transgenic variants, showing in some cases significant
changes that might be employed for identification of genetic modifications of plants or foods of plant
origin. (doi: 10.5562/cca1777)

Keywords

proteins; capillary electrophoresis; dendrimers; polybrene; GMO

Hrčak ID:

74776

URI

https://hrcak.srce.hr/74776

Publication date:

13.11.2011.

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